相关实验视频
Updated: Jul 12, 2025
04:48
Rose Bengal-Mediated Photodynamic Therapy to Inhibit Candida albicans
Published on: March 24, 2022
3.2K
在Candida Albicans上使用Geraniol纳米乳液改善药物输送
Cristiano Silva Pontes1, Gabriel Garcia de Carvalho1, Andressa Rosa Perin Leite2
1Department of Physiology and Pathology, School of Dentistry at Araraquara, São Paulo State University (Unesp), Araraquara 14801-903, SP, Brazil.
Pharmaceutics
|October 28, 2023
概括
格拉尼 (GE) 纳米乳液 (NE) 有效地对抗Candida albicans生物膜,具有显著的抗真菌活性. 这种新型的输送系统显示出治疗真菌感染而不会引起细胞毒性的前景.
科学领域:
- 菌类学 菌类学是指菌类学.
- 纳米技术纳米技术
- 生物化学 生物化学
背景情况:
- 格拉尼醇 (GE) 是一种单烯醇,具有强大的抗真菌特性,但具有不良的溶解性和高挥发性.
- 纳米乳液 (NE) 提供了一种可行的解决方案,用于输送难溶和挥发性化合物,从而实现可控释放和提高效率.
- 菌 (Candida albicans) 是一个重要的机会性真菌病原体,经常形成对常规疗法的耐药生物膜.
研究的目的:
- 开发和表征醇载荷纳米乳液 (GE-NE) 以改善对抗白菌的传递.
- 为了评估GE-NE对Candida albicans的抗菌膜疗效.
- 评估GE-NE在正常口腔细胞上的细胞毒性.
主要方法:
- 纳米乳液是使用胆固醇作为油相合成的,使用大豆酸胆和Brij 58混合物作为表面活性剂,并使用酸盐缓冲剂作为水相.
- 通过超声波实现了将格拉尼奥尔纳入纳米乳液的结合.
- 描述包括水力动力直径,多分散度指数 (PDI),泽塔潜力 (ZP) 和6个月和1年的物理化学稳定性评估. 进行了抗生物膜活性和细胞毒性测试.
主要成果:
- 合成的GE-NE具有均的粒子大小 (232.3 ± 2.7 nm) 和低的PDI (0.155),表明其同质性和稳定性.
- GE-NE显示出针对Candida albicans的显著抗菌膜活性,在75μg/mL度下达到>6.0 log10的降低.
- 在高达150μg/mL的度下,对NOK-Si口服细胞系没有观察到细胞毒性.
结论:
- 带醇的纳米乳液 (GE-NE) 是一种稳定有效的输送系统,可以增强醇的抗真菌特性.
- GE-NE显示出作为预防和治疗Candida albicans生物膜感染的治疗剂的显著潜力.
- 开发的GE-NE配方在有效度下是非细胞毒性的,这表明它对潜在的临床应用具有有利的安全性.
相关概念视频
01:16Drug Delivery: Overview
300
The selection of a drug's delivery route depends upon its physicochemical properties, including lipid or water solubility and ionization, as well as the therapeutic requirement, such as immediate or sustained effect. These routes can be divided into three primary categories: enteral, parenteral, and topical.
Enteral delivery involves administering drugs directly through swallowing, sublingual placement, or buccal application. Orally administered drugs predominantly navigate the...
Enteral delivery involves administering drugs directly through swallowing, sublingual placement, or buccal application. Orally administered drugs predominantly navigate the...
300
01:22Drug Delivery: Miscellaneous Routes
359
Drug delivery methods like oral inhalation, nasal sprays, transdermal patches, eye drops, intravitreal injection, and rectal administration provide localized effects with reduced toxicity.
Oral inhalation and nasal sprays swiftly transfer drugs across the respiratory epithelium's mucosal layer. Inhaled glucocorticoids and bronchodilators directly target lung conditions such as asthma, while fluticasone nasal spray mitigates allergic rhinitis.
Transdermal patches transport drugs...
Oral inhalation and nasal sprays swiftly transfer drugs across the respiratory epithelium's mucosal layer. Inhaled glucocorticoids and bronchodilators directly target lung conditions such as asthma, while fluticasone nasal spray mitigates allergic rhinitis.
Transdermal patches transport drugs...
359
01:18Drug Delivery: Enteral Route
468
The enteral drug administration involves three primary routes: oral, sublingual, and buccal. Oral ingestion is the most prevalent, safe, economical, and convenient method for drug administration. However, it has certain drawbacks, including limited absorption due to the drug's low water solubility or poor membrane permeability, possible emesis from GI mucosa irritation, destruction of drugs by digestive enzymes or low gastric pH, and irregular absorption along with food or other drugs.
468
01:23Factors Affecting Dissolution: Particle Size and Effective Surface Area
864
Dissolution kinetics, an essential aspect of oral drug delivery, is significantly influenced by the drug's particle size. According to the Noyes-Whitney dissolution model, the dissolution rate correlates directly with the drug's surface area. The larger the surface area, the higher the drug's solubility in water, leading to a faster drug dissolution rate. Reducing particle size increases the effective surface area, enhancing the dissolution process. Micronization and nanosizing are...
864