多孔聚二乙基甲酸) 组织工程的水凝支架:交叉连接系统和丝素度对支架性能的影响
Nantaprapa Tuancharoensri1, Sukhonthamat Sonjan1, Sudarat Promkrainit1
1Biopolymer Group, Department of Chemistry, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand.
Polymers
|October 28, 2023
概括
这项研究开发了新的多孔水凝支架,使用聚二乙基甲酸 (PHEMA) 和丝素 (SS) 两种交叉连接系统. 开发出来的支架模仿了细胞外基质,并且由于其非细胞毒性特性,显示了组织工程的潜力.
科学领域:
- 生物材料科学 生物材料科学
- 聚合物化学 聚合物化学
- 组织工程是组织工程.
背景情况:
- 细胞外基质 (ECM) 提供了细胞功能所必需的结构支持和生化线索.
- 模仿ECM的多孔结构对于开发有效的组织工程支架至关重要.
- 聚二乙基甲酸 (PHEMA) 和丝素 (SS) 是具有支架制造潜力的生物相容材料.
研究的目的:
- 使用PHEMA和SS创建定制的多孔水凝支架,具有独特的交叉连接系统.
- 研究化学 (MBAAm) 和物理 (DMU) 交联对脚手架性能的影响.
- 评估这些支架在组织工程应用中的潜力.
主要方法:
- 使用自由基聚合HEMA与SS的不同度的多孔水凝支架的制造.
- 采用两种交叉连接系统:用N,N'-甲基基亚胺 (MBAAm) 的化学交叉连接和用二甲基尿素 (DMU) 的物理交叉连接.
- 脚手架属性的表征包括毛孔大小,形态,降解,热行为和体外细胞毒性.
主要成果:
- 与MBAAm和DMU (C-PHEMA/pC-SS) 交叉连接的支架相比,与仅与MBAAm (C-PHEMA/SS) 交叉连接的支架具有更小,更规律的孔径.
- 增加的SS度导致毛孔尺寸进一步减少.
- 由于DMU的存在,C-PHEMA/pC-SS支架显示出延迟的凝时间.
- 这两种支架类型都对正常人皮肤皮肤纤维细胞 (NHDF) 细胞表现出非细胞毒性.
结论:
- 交叉连接系统的选择显著影响PHEMA/SS水凝支架的多孔结构和特性.
- 在C-PHEMA/pC-SS支架提供可调节的孔特性,适合模仿本地ECM.
- 这些非细胞毒性水凝支架对未来的组织工程应用具有前景.
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