相关实验视频

Updated: Jul 12, 2025

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

Published on: January 24, 2016

一种基于蛋白质学的方法将NEDD4适配器NDFIP2确定为Ifitm3水平的重要调节器

Federico Marziali¹, Yuxin Song¹, Xuan-Nhi Nguyen¹

¹Centre International de Recherche en Infectiologie (CIRI), Univ Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, 69100 Lyon, France.

|October 28, 2023

概括

干扰素诱导的跨膜蛋白 (IFITM) 具有广泛的抗病毒特性. 这项研究确定NDFIP2是IFITM3水平的关键调节者,影响病毒防御机制.

科学领域:

病毒学病毒学
细胞生物学细胞生物学
蛋白质组学是指蛋白质组学.

背景情况:

干扰素诱导的跨膜蛋白 (IFITM) 通过抑制病毒和细胞膜融合,对广泛的抗病毒防御至关重要.
IFITM的抗病毒疗效取决于蛋白质水平,细胞局部化和翻译后的修改,受各种细胞合作伙伴的影响.

研究的目的:

确定调节干扰素诱导的跨膜蛋白3 (IFITM3) 生物学的新型蛋白合作伙伴.
阐明NDFIP2蛋白适配器在调节IFITM3水平和功能的作用.

主要方法:

基于蛋白质组学的方法使用细胞外囊泡来识别IFITM3相互作用蛋白.
囊泡蛋白质组的质谱 (MS) 分析.
基因操纵 (不激活和过度表达) 的NDFIP2.2.

主要成果:

NDFIP2被确定为IFITM3水平的关键调节者.
NDFIP2作为IFITM3和NEDD4的招募因子,调解它们的溶酶体分布.
NDFIP2调节IFITM3的积累,这表明对NEDD4的约束有竞争.

结论:

NDFIP2代表了IFITM3的新型监管层,影响其稳定性和抗病毒活性.
NDFIP2,IFITM3和NEDD4之间的相互作用为抗病毒反应的动态控制提供了新的见解.

关键词:

如果IFITM3是IFITM3,那么它是什么?在 NDFIP2 的基础上.在NEDD4中,它是NEDD4.干扰子干扰子干扰子病毒病毒病毒病毒

更多相关视频

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

Published on: October 6, 2019

相关概念视频

Regulation of the Unfolded Protein Response

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...