在的巨中,B细胞激活因子转录的长度依赖调节由病毒双链RNA进行
Magda I Abo-Samaha1,2, Mohammed M Sharaf2, Abeer F El-Nahas2
1Department of Pathobiology, College of Veterinary Medicine, Tuskegee University, Tuskegee, AL 36830, USA.
Vaccines
|October 28, 2023
概括
的巨缺少RIG-1,但对长dRNA做出反应,对细胞因子进行上调. 短dRNA可以通过调节BAFF来抑制B细胞的发育,独立于1型干扰素反应.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 分子生物学分子生物学
背景情况:
- 病毒双链RNA (dsRNA) 通过视网膜酸可诱导基因-1 (RIG-1) 类受体 (RLRs) 的识别对于1型干扰素诱导至关重要.
- 表达黑色素瘤相关抗原5 (MDA-5),但缺乏RIG-1,不像子,这可能解释了差异性流感易感性.
- 了解的dsRNA传感对于禽类免疫学和疾病耐药性至关重要.
研究的目的:
- 为了研究巨对不同长度的多I:C的差异性免疫反应,dsRNA替代物.
- 阐明MDA-5的作用和RIG-1在dRNA感知通路中的缺失.
- 为了确定短dRNA对B细胞发育和生存的影响,在的巨细胞激活的背景下.
主要方法:
- 利用HD11巨细胞系进行体外实验.
- 使用RT2 Profiler PCR-array系统来分析mRNA表达特征.
- 用于高分子量 (HMW) 和低分子量 (LMW) 聚 (I:C) 刺激巨细胞.
主要成果:
- HMW-poly(I:C) 显著上调了CCL4,干扰素-,介质素-1,介质素-6,IL-12B和B细胞激活因子 (BAFF) 的mRNA转录.
- 单独的LMW-poly (I:C) 并没有诱导明显的细胞因子模式.
- 与LMW和HMW-poly共同感染 (I:C) 抑制了HMW-poly (I:C) 诱导IL12B和BAFF的上调.
结论:
- 缺乏RIG-1的巨通过上调特定细胞因子来响应长dRNA (HMW-poly(I:C)).
- 短dSRNA (LMW-poly(I:C)) 可能通过BAFF调节在巨中抑制B细胞的发育和生存,而不会显著影响1型干扰素反应.
- 这些发现突出了中独特的dsRNA感知机制,可能会影响它们对病毒感染的免疫反应.
关键词:
巴夫 (BAFF) 是一个巴夫.HD11 HD11 HD11 HD11 HD11 HD11 HD11 HD11 HD11 HD11这些都是肉肉.dsRNARNA 是一个巨细胞是一个巨细胞.聚I:C) 是一个多I:C) 是一个多I:C) 是一个多I:C) 是一个多I:C) 是一个多I:C).更多相关视频
05:03Author Spotlight: Elucidating the Pathways of TFH Cell Differentiation in Acute LCMV Challenges
Published on: April 26, 2024
716
07:17Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice
Published on: June 22, 2016
9.9K
相关概念视频
02:28Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
01:29Immune Response Against Viral Pathogens
798
The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
798
01:24B Cell Activation and Differentiation
1.7K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
1.7K