来自Candida antarctica的脂酶B在两的Janus halloysite纳米板上固定,并在双相接口转换中的应用
Lili Yin1, Kunpeng Gao1, Xiangzhao Mao2
1Qingdao Key Laboratory of Food Biotechnology, College of Food Science and Engineering, Ocean University of China, Qingdao 266404, PR China; Key Laboratory of Biological Processing of Aquatic Products, China National Light Industry, Qingdao 266404, PR China.
Food chemistry
|October 28, 2023
概括
这项研究开发了Janus halloysite nanosheets (JHNS) 来固定Candida antarctica Lipase B (CALB) 以实现高效的双相生物转化. 在JHNS-CALB系统显著提高了酶性能和转化效率有价值的脂质.
科学领域:
- 生物催化和酶固定化
- 材料科学和纳米技术材料科学和纳米技术
- 绿色化学 绿色化学
背景情况:
- 来自Candida antarctica (CALB) 的B脂酶是工业脂质转化的一个关键生物催化剂.
- 双相系统对于功能性脂质转换至关重要,但在稳定性和效率方面面临挑战.
- 开发强大的酶固定化策略对于提高生物催化剂性能至关重要.
研究的目的:
- 制造两性Janus化酸盐纳米薄膜 (JHNS) 用于稳定皮克林乳液.
- 利用JHNS作为一个载体来固定CALB,旨在实现高效的双相生物转换.
- 在稳定性和催化活性方面评估JHNS固定CALB的性能.
主要方法:
- 制造的两性类化体纳米片 (JHNS).
- 在JHNS上对Candida antarctica Lipase B (CALB) 的固定.
- 使用JHNS稳定皮克林乳液.
- 评估固定CALB的稳定性和催化效率.
- 在二相转化中的应用 乙乙.
主要成果:
- JHNS有效地稳定了Pickering乳液超过7天.
- 在JHNS上CALB的固定增强了基质亲和力,催化效率,热稳定性和性耐受性.
- 基于JHNS的固定CALB显示,其转换效率几乎是网酸乙烯的两倍.
- 开发的系统为双相生物转化提供了一个稳定高效的平台.
结论:
- 简氏化体纳米片为CALB固定和皮克林乳液稳定提供了稳定有效的平台.
- 通过JHNS固定化的CALB具有卓越的酶特性,并显著提高了转化效率.
- 这种方法代表了开发高级生物催化系统的有希望的战略,用于增值脂质生产.
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