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相关概念视频

Glycolysis: Preparatory Phase01:21

Glycolysis: Preparatory Phase

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In cellular metabolism (the complete breakdown of glucose to extract energy),  glycolysis is the first step. Glycolysis takes place in the cytoplasm of both prokaryotic and eukaryotic cells. Glucose enters heterotrophic cells in two ways. One method is through secondary active transport, where the transport takes place against the glucose concentration gradient. The other mechanism uses a group of integral proteins called GLUT proteins, also known as glucose transporter proteins. These...
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Fates of Pyruvate01:20

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Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
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Glycolysis01:23

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Glycolysis, the Embden-Meyerhof pathway, is a central metabolic pathway involved in glucose catabolism. It is highly conserved across most organisms, reflecting its fundamental role in cellular energy production. This process occurs in the cytoplasm and can function both in the presence and absence of oxygen, making it versatile for various organisms and environmental conditions.Stages of GlycolysisGlycolysis is a ten-step pathway that converts glucose into pyruvate, generating a net gain of...
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ATP Energy Storage and Release01:31

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ATP is a highly unstable molecule. Unless quickly used to perform work, ATP spontaneously dissociates into ADP and inorganic phosphate (Pi), and the free energy released during this process is lost as heat. The energy released by ATP hydrolysis is used to perform work inside the cell and depends on a strategy called energy coupling. Cells couple the exergonic reaction of ATP hydrolysis with endergonic reactions, allowing them to proceed.
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Other Glycolytic Pathways01:24

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The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
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Energy-requiring Steps of Glycolysis01:20

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Glucose is the source of nearly all energy used by organisms. The first step of converting glucose into usable energy is called glycolysis. Glycolysis occurs in the cytosol of the cell over two phases: an energy-requiring phase and an energy-releasing phase. Over the first three steps, glucose is converted into different forms and attached to two phosphate groups donated by two ATP molecules, resulting in an unstable sugar. In the next two stages, the unstable sugar splits into two sugar...
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使用葡萄糖去除有氧颗粒污泥中的酸盐.

Ali Elahinik1, Linghang Li1, Martin Pabst1

  • 1Department of Biotechnology, Delft University of Technology, van der Maasweg 9, Delft 2629HZ, the Netherlands.

Water research
|October 28, 2023
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概括

有氧颗粒污泥有效地使用可发酵基质葡萄糖去除酸盐. 这项研究证明了葡萄糖的存在.

关键词:
有氧颗粒污泥的有氧颗粒污泥这是因为. 积累了一些细菌.增强了生物去除的功能.发酵性GAO是一种GAO.微型蛋白质 (Micropruina) 是一种微型蛋白质.蛋白质组学是指蛋白质组学.

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科学领域:

  • 环境生物技术 环境生物技术
  • 废水处理 废水处理
  • 微生物生态学 微生物生态学

背景情况:

  • 有氧颗粒污泥 (AGS) 对于增强的生物酸盐去除 (EBPR) 是至关重要的.
  • 脂肪酸是EBPR的常见基质,但像葡萄糖这样的可发酵基质研究较少.
  • 有限的研究存在于AGS的葡萄糖转化及其对酸盐去除效率的影响.

研究的目的:

  • 为了研究有氧颗粒污泥的葡萄糖转化.
  • 评估葡萄糖作为基质对生物酸盐去除的影响.
  • 描述在AGS中参与葡萄糖代谢的微生物群体和代谢途径.

主要方法:

  • 使用有氧颗粒污泥作为唯一碳来源的葡萄糖进行长期实验.
  • 监测酸盐的吸收/释放,基质消耗和副产品的形成.
  • 定量光现场杂交 (qFISH) 和元基因组分析以确定微生物种群 (例如,PAO,Micropruina,Ca. 积聚的细菌).

主要成果:

  • 使用葡萄糖实现了稳定的酸盐去除和成功的有氧颗粒化.
  • 葡萄糖迅速无氧消耗,导致酸盐的释放和随后的吸收.
  • 乳酸是主要的发酵产物,与酸盐释放直接相关;观察到多基酸盐和糖原储存.

结论:

  • 有氧颗粒污泥技术对于处理含有葡萄糖的废水是有效的,葡萄糖支持有效的酸盐去除.
  • 在可发酵基质条件下,由发酵生物和聚酸盐积累生物 (PAO) 组成的微生物群体会发展.
  • 该研究证实葡萄糖是AGS系统中EBPR的合适基质,强调了微生物联盟的适应性.