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在汉语中对短期,中期和长期臭氧暴露进行表观基因组范围的关联研究,NSPT研究

Xiyang Cai¹, Kaixuan Li¹, Xia Meng²

¹CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.

Journal of hazardous materials

|October 28, 2023

概括

环境臭氧暴露与健康问题有关. 这项研究揭示了与臭氧相关的中国成年人的特定DNA甲基化变化,影响了与衰老和疾病相关的基因.

科学领域:

环境表观遗传学环境表观遗传学
分子流行病学分子流行病学

背景情况:

流行病学和表观遗传学研究将环境臭氧暴露与炎症和心血管疾病联系起来.
分子机制和整个表观基因组的分析,特别是在中国人群中,仍未得到充分研究.

研究的目的:

进行第一个全表观基因组关联研究 (EWAS) 关于大规模汉族中华队列中臭氧暴露.
为了确定与短期,中期和长期臭氧暴露相关的DNA甲基化变化.
探索已识别的甲基化变化对基因和通路的功能影响.

主要方法:

包括来自NSPT队列中的3365名中国参与者的血源DNA甲基化数据.
根据不同时间窗口的验证预测模型估计的个人臭氧暴露水平.
进行了全表观基因组关联研究 (EWAS),以确定显著的CpG和差异甲基化区域 (DMR).

主要成果:

在全基因组显著性 (P < 5 × 10−8) 确定了与臭氧暴露相关的59个CpG和30个DMR.
观察到与短期暴露相比,中长期暴露于臭氧的差异甲基化趋势更强.
向基因与衰老,炎症,代谢综合征,神经发育障碍和瘤发生有关.
丰富的途径包括端粒维护,DNA损伤反应和巨核细胞分化.

结论:

这项研究是第一个关于大汉族中国人群中臭氧暴露的EWAS研究.
确定了臭氧暴露与CpG和区域的DNA甲基化变化之间的强有力的关联.
揭示了潜在的分子通路,将臭氧暴露与各种健康结果联系起来.

关键词:

通过DNA甲基化.欧洲经济联盟 (EWAS) 的成员.功能分析是一种功能分析.臭氧臭氧是什么?臭氧臭氧是什么路径丰富方式

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