相关实验视频

Updated: Jul 12, 2025

Isolation of Epithelial Cells from Human Dental Follicle

Isolation of Epithelial Cells from Human Dental Follicle

Published on: November 5, 2021

从牙上皮质到牙上皮质的AKT促进了口腔细胞分化

Jiangyi Wang¹, Xiaoyu Lin¹, Zongshan Shen¹

¹Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Beijing Laboratory of Oral Health and Beijing Stomatological Hospital, Capital Medical University, Beijing, 100050, China.

Differentiation; research in biological diversity

|October 28, 2023

概括

牙上皮质 (DE) 中的酸-3-酶 (PI3K) /AKT通路向牙上皮质 (DP) 细胞发出信号,使其分化为牙细胞. 这一过程涉及原4型IVα6链 (COL4A6) 的分泌,这对牙发育至关重要.

科学领域:

发展生物学发展生物学
细胞信号传递细胞信号传递
牙的生长过程 (Odontogenesis)

背景情况:

表皮-介质酶相互作用对牙发育至关重要.
牙上皮 (DE) 作为一个调节牙形态的信号中心.
对于DE如何影响牙皮 (DP) 分化为牙细胞的机制,尚不完全理解.

研究的目的:

阐明表皮层-介质细胞信号在芽细胞分化中的作用.
调查酸丁-3-酶 (PI3K) /AKT通路在这个过程中的参与.
在牙发育过程中识别DE-DP通信的特定分子媒介.

主要方法:

使用高通量RNA测序来分析微型猪的DE和DP中的基因表达特征.
分析的重点是牙细胞分化期间的关键时间点 (胚胎日期50和60).
研究PI3K/AKT通路和IV型原蛋白α6链 (COL4A6) 的激活模式和功能影响.

主要成果:

在DE和DP中,PI3K/AKT通路被显著丰富,最初在内面膜上皮质中激活.
AKT的激活被证明可以促进DP细胞的牙细胞分化.
在DP细胞中,PI3K/AKT信号的DE激活增加了AKT和牙光蛋白的表达,这种表达是由COL4A6分泌的介导.

结论:

PI3K/AKT通路是在口腔细胞分化过程中,是DE向DP的关键信号机制.
由DE细胞分泌的IV型原蛋白α6链 (COL4A6),在这种信号级联中起着关键的调解作用.
这项研究揭示了一种涉及DE衍生的COL4A6的新途径,该途径通过中酶体AKT激活促进了口腔细胞分化.

关键词:

细胞分化的细胞分化.细胞相互作用细胞相互作用培养培养培养牙的乳头是指牙的乳头.发展发展发展发展发展牙的牙是一个牙.

更多相关视频

Accessing the Cytotoxicity and Cell Response to Biomaterials

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy

Published on: April 13, 2022

相关概念视频

Cellular Differentiation

Cellular Differentiation

How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array of cell types, such as nerve cells, muscle cells, and epithelial cells that characterize the adult? Throughout development and adulthood, cellular differentiation leads cells to assume their final morphology and physiology. Differentiation is the process by which unspecialized cells become specialized to carry out distinct functions.
A zygote is a...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...