叶绿素及其在化后形成的主要转化产品的神经毒性作用
Shengchao Hu1, Jian Zhao1, Shangbiao Fang1
1Center for Water and Ecology, State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China.
The Science of the total environment
|October 28, 2023
概括
废水处理中的化物转化产品 (TPs) 构成环境风险. 一种主要的TP,化TP353,在斑马鱼胚胎中显示出比洛昆本身更大的神经毒性.
科学领域:
- 环境化学环境化学
- 生态毒理学 生态毒理学
- 制药科学 制药科学
背景情况:
- 废水中的药品是一个新兴的环境问题.
- 关于废水处理过程中制药降解的转化产品 (TPs) 的知识有限.
- 用于COVID-19的Chloroquine (CQ) 是一个相关的药物来研究.
研究的目的:
- 从消毒中识别和分离主要的CQ TP.
- 评估CQ及其主要TP对斑马鱼胚胎的神经毒性影响.
- 评估制药TP的环境风险.
主要方法:
- (CQ) 的消毒.
- 识别和分成部分的CQ TPs.
- 斑马鱼 (Danio rerio) 的胚胎毒性测试.
- 基因表达分析 (gfap,syn2a,elavl3) 的方法.
- 乙胆酶 (AChE) 活性测定.
- 在转基因幼虫 (hb9-GFP) 中进行运动神经元轴突成像.
- 行为分析 (游泳距离和速度).
主要成果:
- 化TP353被确定为主要的CQTP.
- 在斑马鱼幼虫中,TP353的神经毒性高于CQ.
- 暴露在TP353下降了中枢神经系统发育基因的表达.
- TP353 抑制了 AChE 活动和运动神经元轴突完整性.
- 幼虫游泳行为受到TP353.3的影响.
结论:
- TP353对水生生物构成显著的神经毒性风险.
- 在废水处理后,CQ TP 构成潜在的环境危害.
- 方法论为评估药物TP风险提供了一个框架.
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