转录因子STAT增强了Bombyx mori中的抗菌活性
Li Zhou1, Zhuo Dang1, Shiyuan Wang1
1Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, China; Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Chongqing 400715, PR China.
丝虫STAT (信号转换器和转录激活器) 蛋白质,STAT-S和STAT-L,促进抗微生物基因表达,增强对细菌和真菌病原体的耐药性. 这项研究澄清了STAT.
科学领域:
- * 昆虫免疫学 昆虫免疫学
- * 分子生物学 * 分子生物学
- * JAK/STAT信号通道的使用
背景情况:
- *信号传感器和转录激活剂 (STAT) 蛋白调节对病原体的免疫反应.
- * 丝虫 (Bombyx mori) 具有两个STAT分裂形式:STAT-S和STAT-L.
- *STAT在丝虫免疫力中的确切作用需要阐明.
研究的目的:
- * 调查STAT对抗微生物 (AMP) 基因表达的影响.
- * 确定STAT对丝虫对病原体耐药性的影响.
主要方法:
- * 在微生物诱导后对STAT-S和STAT-L表达的量化.
- * 针对免疫刺激 (LPS,PGN,β-葡萄糖) 的反应中,STAT转移到核的分析.
- *转基因丝虫产生和评估STAT-S/STAT-L过度表达或干扰 (OE-STAT-S,OE-STAT-L,A4-dsSTAT).
主要成果:
- * 病原体诱导显著上调了STAT-S和STAT-L表达.
- *STAT-S和STAT-L在刺激后转移到核中,并促进AMP基因表达.
- *过度表达STAT-S/STAT-L增强了AMP表达和改善了丝虫对抗S. aureus和B. bassiana感染的生存率,而STAT干扰增加了死亡率.
结论:
- *STAT-S和STAT-L在丝虫的抗菌免疫反应中起着至关重要的作用.
- *STAT蛋白增强抗菌的表达,增强宿主防御.
- * 结果提供了关于JAK/STAT通路在昆虫免疫中的功能意义的见解.
更多相关视频
05:20Hyperactive piggyBac Transposase-mediated Germline Transformation in the Fall Armyworm, Spodoptera frugiperda
Published on: September 23, 2021
09:25Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
Published on: June 2, 2021
相关概念视频
Master Transcription Regulators
General Transcription Factors
Combinatorial Gene Control
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
Transcription Attenuation in Prokaryotes
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
Eukaryotic Transcription Inhibitors
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
RNA Polymerase II Accessory Proteins
