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将RNA测序,全外体测序和流细胞测量整合到儿科淋巴瘤的常规诊断工作中

Marijn A Scheijde-Vermeulen¹, Lennart A Kester¹, Liset Westera¹

¹Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.

Laboratory investigation; a journal of technical methods and pathology

|October 28, 2023

概括

将高级测序和流细胞测量整合到儿科淋巴瘤诊断中是可行的. 这些方法提高了诊断的准确性,并支持儿童淋巴瘤的未来治疗策略.

科学领域:

在瘤学瘤学.
遗传学遗传学是一个
血液学血液学血液学

背景情况:

儿童淋巴瘤的诊断依赖于传统的方法.
需要先进的技术来提高诊断准确度,并确定新的治疗点.

研究的目的:

评估将下一代测序 (NGS) 和流细胞计纳入儿科淋巴瘤常规诊断工作流程的可行性.
评估这些先进技术在诊断儿科淋巴瘤和识别遗传异常方面的附加值.

主要方法:

在儿科淋巴瘤活检的诊断工作中实施RNA测序 (RNAseq),全外体测序和流细胞计.
在一年内对110名儿童的122个样本进行了分析,结合了组织学,免疫组织化学,流细胞计和NGS.

主要成果:

在83%的新鲜标本中,流细胞计能够快速诊断T细胞和B细胞非霍奇金淋巴瘤.
RNAseq检测到大多数转位,并确定了传统方法遗漏的额外遗传异常.
技术和专家之间的协同作用促进了全面的诊断.

结论:

集成先进的测序和流细胞计是可行的,并为儿科淋巴瘤诊断增加了显著的价值.
这种综合方法支持准确的诊断,并为开发儿童淋巴瘤的新型基于研究的治疗策略奠定了基础.

关键词:

的DNA和RNA测序.流动细胞计量是流动细胞计量的方法.淋巴瘤诊断淋巴瘤诊断淋巴瘤诊断儿科淋巴瘤儿童淋巴瘤

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相关概念视频

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...