从原始细胞生成的长期可活的仿制性子是西斯胺诱导毒性的可靠模型
Kenji Matsui1, Shuichiro Yamanaka2, Sandy Chen1
1Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, 105-8461, Japan.
Communications biology
|October 29, 2023
概括
新生儿注射前代细胞会产生与宿主尿道集成的模拟性子,从而实现长期活力和功能. 这种方法为脏再生和药物查提供了一个有希望的方法.
科学领域:
- 再生医学是一种再生医学.
- 发展生物学 发展生物学
- 腎臟病學 (nephrology) 是一種醫學專業.
背景情况:
- 器官体在体外成熟度有限,缺乏尿路整合,无法长期生存.
- 以前的成年小鼠移植方法由于缺乏整合,无法实现长期可行的产生的脏.
- 证明长期可行的,功能性脏对于推进脏研究和治疗至关重要.
研究的目的:
- 开发一种可靠的方法,在体内产生长期可行的功能性脏.
- 为了研究宿主脏内的异种移植原细胞的整合和成熟.
- 建立一个研究损伤和再生的模型.
主要方法:
- 在新生小鼠脏中注射小鼠和老鼠脏前代细胞.
- 嵌合体神经元的生成与集成到宿主泌尿道.
- 使用单细胞RNA测序评估脏成熟,活力,功能 (分泌/再吸收) 和对西斯普拉丁诱导的损伤的反应.
主要成果:
- 成功生成了与宿主泌尿道集成的嵌合性子.
- 化学性脏表现出与宿主脏和长期生存能力相似的成熟.
- 观察到功能性分泌和再吸收能力,以及对急性和慢性思普拉丁损伤的反应.
- 人类脏原始细胞也在新生儿脏中分化为脏.
结论:
- 新生儿注射前代细胞是体内子生成的一个有前途的方法.
- 这种方法促进了产生的脏细胞的长期生存能力和功能整合.
- 潜在的应用包括脏再生,药物查和病理分析.
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