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CRH-R2信号调节了海马体mHypoA-2/30神经元中的食和昼夜基因表达

Viridiana Alcántara-Alonso^1,2, Robert Dallmann¹, Hendrik Lehnert³

¹Translational Medicine, Warwick Medical School, University of Warwick, Coventry, United Kingdom.

Frontiers in endocrinology

|October 30, 2023

概括

这项研究揭示了下丘脑CRH-R2信号如何影响食欲和昼夜节律,特别是在压力下. 乌洛科丁2增强了Bmal1的表达,可能有助于在活跃的昼夜阶段对厌食反应.

科学领域:

神经内分泌学神经内分泌学
代谢调节代谢调节代谢调节代谢调节
时间生物学时间生物学

背景情况:

脑下垂体皮质激素释放激素受体2 (CRH-R2) 对于压力恢复和食欲控制至关重要.
慢性压力可能导致CRH-R2抵抗,改变养行为和信号通路,如CREB酸化.
这些压力诱导的CRH-R2信号传递变化背后的分子机制仍然不清楚.

研究的目的:

在小鼠下丘脑衍生细胞系 (mHypoA-2/30) 中研究CRH-R2信号传递.
检查CRH-R2对养和昼夜调节基因表达的影响.
阐明乌罗皮素2 (Ucn2) 对时钟基因和CRH-R2信号传递的影响.

主要方法:

利用mHypoA-2/30细胞系研究CRH-R2信号传递.
评估了CREB和AMPK的酸化.
分析了食欲调节基因 (Pomc,Npy) 和时钟基因 (Bmal1) 的表达.
雇佣昼夜生物发光记者来监测时钟基因节律.

主要成果:

在mHypoA-2/30细胞中确定了功能性的CRH-R2受体.
CRH-R2激活不同调节的CREB和AMPK酸化.
CRH-R2信号调节了Pomc和Npy的表达,与厌氧效应一致.
在原生细胞和记者细胞中,ucn2增强了bmal1mRNA水平和节律幅度.

结论:

下丘脑中的CRH-R2信号通过Pomc和Npy影响食欲调节.
Ucn2对Bmal1的影响表明它在压力期间的昼夜食调节中发挥了作用.
这些发现为下丘脑CRH-R2信号传递在昼夜养和应激反应的背景下提供了新的见解.

关键词:

这就是AMPKK.克里布 (Creb) 是一个有趣的词汇.在CRH-R2中使用.养养养养昼夜节律昼夜节律脑下垂体中的类.泌尿皮质素2 (UCN2) 是一种

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