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在无溶剂系统中,在多孔基材料上吸附脂酶以进行化
Kelly C N R Pedro1, João V V da Silva1, Eliane P Cipolatti2
1Departamento de Química Analítica, Instituto de Química, Universidade do Estado do Rio de Janeiro (UERJ), Rua São Francisco Xavier, 524, 20550-900 Rio de Janeiro, RJ Brasil.
3 Biotech
|October 30, 2023
概括
像Siral 20和40这样的基于的材料有效地固定脂质酶,增强它们的稳定性和乙烯酸合成的活性. 这些固定生物催化剂在多个反应周期中显示出良好的可重复使用性.
科学领域:
- 生物催化和酶固定化
- 材料科学 材料科学 材料科学
- 化学工程是化学工程的重要组成部分.
背景情况:
- 脂酶是各种工业应用的关键酶,但它们的自由形式往往遭受不稳定和困难的恢复.
- 将脂酶固定在固体支上是提高其可重复使用性,稳定性和催化效率的关键策略.
- 基于的材料,包括化石和化,由于它们的高表面积和可调节的多孔性,为酶固定提供了有前途的支持性质.
研究的目的:
- 研究来自南极 (Candida antarctica) 的脂酶B (CALB) 和来自Rhizomucor miehei (RML) 的脂酶在各种微型和中等孔状基材料上的固定效率.
- 评估支持性质,蛋白质度,pH和缓冲度对脂酶固定化的影响.
- 评估乙烯酸盐合成中的固定脂酶的性能,热稳定性和可重复使用性.
主要方法:
- 将CALB和RML固定在MCM-41的二氧化,HZSM-5的化物 (SAR 25,SAR 280) 和二氧化 (Siral 10,20,40) 上.
- 使用兰迈尔异热模拟的固定效率和蛋白质吸附的表征.
- 在不同pH值和缓冲度下对固定性进行研究.
- 使用固定生物催化剂 (CALB-Siral 40,RML-Siral 20) 进行乙烯酸盐合成的测试,并评估热稳定性和可重复使用性.
主要成果:
- 西拉尔40号,HZSM-5号 (SAR280号) 和MCM-41号显示了CALB的高固定效率 (高达91.4%).
- 锡拉尔20显示了对RML的最高固定效率 (97.6%),其次是HZSM-5 (SAR 25) (77.1%).
- 兰迈尔异热模型准确地描述了蛋白质吸附,CALB的最大容量为10.64 mg/g,RML的最大容量为20.97 mg/g.
- 固定效率在很大程度上不受pH的影响,但随着缓冲度的提高 (100 mmol L-1),缓冲效率下降.
- 在60°C时,CALB-Siral 40在乙烯酸盐合成中实现了61.7%的转化,两种生物催化剂都显示出增强的热稳定性.
- 在3个反应周期后,固定化脂酶保留了大约30%的初始转化.
结论:
- 合金 (Siral 20和40) 是高效脂酶固定化的有效支持物.
- 固定化脂酶表现出更好的热稳定性和可重复使用性,使其适合工业化化工艺.
- 这项研究强调了定制的基材料在开发强大的和高效的生物催化剂方面的潜力.
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