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Hs1Cas12a和Ev1Cas12a在植物中提供了高效的基因组编辑

Gen Li¹, Yingxiao Zhang¹, Micah Dailey¹

¹Department of Plant Science and Landscape Architecture, University of Maryland, College Park, College Park, MD, United States.

Frontiers in genome editing

|October 30, 2023

概括

研究人员确定了两个新的CRISPR-Cas12a (Cpf1) 酶,Ev1Cas12a和HS1Cas12a,用于高效的植物基因组编辑. 这些新的工具显示出编辑单和双物种的前景,扩大了基因编辑应用.

科学领域:

分子生物学分子生物学
生物技术是生物技术.
植物科学植物科学

背景情况:

CRISPR-Cas12a (Cpf1) 是一种多功能基因组编辑工具,在富含AT的地区特别有效.
将CRISPR-Cas12a工具箱扩展到新型的正义词对各种植物应用至关重要.

研究的目的:

发现和描述用于植物基因组编辑的新Cas12a基因组.
评估Cas12a新变种在单和双物种中的效率和特征.

主要方法:

为基因组编辑活动选了17个新的Cas12a正义词.
在大米和西红原生质中测试选定的正统物质 (Ev1Cas12a,Hs1Cas12a).
评估T0植物 (大米,) 中的编辑效率,双基编辑和嵌合体.

主要成果:

Ev1Cas12a和Hs1Cas12a在大米和西红原生质中展示了高效的多重基因组编辑.
Hs1Cas12a在较低的温度下表现更好,在米中实现了87.5%的双基编辑.
这两种酶都有效地编辑了T0植物,达到100%的编辑植物,尽管具有很高的虚构主义.

结论:

Ev1Cas12a和Hs1Cas12a是单 (大米) 和双 (西红,) 植物的强大基因组编辑工具.
这些新的Cas12a正义词显著丰富了植物基因工程的可用工具包.
这些发现表明Ev1Cas12a和Hs1Cas12a在各种植物物种中的广泛适用性以及未来的生物技术进步.

关键词:

在Cas12a中,一个正统的 ортолог.在 Ev1Cas12a 中.这就是Hs1Cas12a.基因组编辑基因组编辑低温耐受性耐低温耐受性这是一棵树,树.米米饭米饭米饭米饭.在这里,我们可以看到茄,番茄,番茄.

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