内微生物群落概况和相关代谢物TNBC的变化
Yi Wang1,2,3, Dingding Qu1,2,3, Yali Zhang1,2,3
1Department of Pathology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, China.
Frontiers in oncology
|October 30, 2023
概括
三重阴性乳腺癌 (TNBC) 研究显示,Turicibacter等特定微生物在TNBC瘤中更为丰富. 这些发现将瘤微生物组与代谢变化和潜在的免疫反应联系起来,提供新的诊断和治疗途径.
科学领域:
- 分子病理学分子病理学
- 微生物组研究 微生物组研究
- 癌症新陈代谢 癌症新陈代谢
背景情况:
- 三阴性乳腺癌 (TNBC) 由于治疗选择有限和预后不佳,给健康带来了重大挑战.
- 新兴研究强调了瘤内微生物群落和代谢变化的癌症发展的影响.
- 微生物群影响TNBC的确切机制在很大程度上仍未确定.
研究的目的:
- 研究各种乳腺癌亚型中的瘤内微生物群落的组成.
- 探索与这些微生物群落相关的代谢差异.
- 确定TNBC的微生物组,代谢资料和临床病理特征之间的相关性.
主要方法:
- 16S rRNA MiSeq测序的甲固定,嵌 (FFPE) 乳腺癌组织样本.
- 基于质谱的代谢分析以确定关键代谢物.
- 微生物种群,代谢物,临床病理学数据和瘤透淋巴细胞 (TILs) 之间的相关性分析.
主要成果:
- 在TNBC样本中观察到显著更高的Turicibacter丰度.
- 确定了不同的代谢概况,包括营养醇,孕妇醇和皮质醇的变化.
- 在瘤内微生物群,临床病理学特征和HER2表达之间发现的相关性.
- 特定的微生物种群 (Cytophagaceae,Conexibacteraceae,Flavobacteriaceae) 与TILs和抗瘤免疫有关.
结论:
- 可以有效地使用FFPE样本来研究内微生物组及其在乳腺癌中的代谢相关性.
- 鉴定的微生物和代谢特征为TNBC中的新型诊断生物标志物提供了潜力.
- 研究结果表明,针对微生物群的新治疗策略可以改善TNBC治疗和早期诊断.
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