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一个独特的细胞群体表达了表皮细胞-介质细胞过渡转录因子,牛调节了微质和天体细胞损伤反应

Cheryl Clarkson-Paredes^1,2, Molly T Karl¹, Anastas Popratiloff^1,2

¹Department of Anatomy and Cell Biology, School of Medicine and Health Sciences, George Washington University, 2300 Eye Street NW, Ross 735, Washington, DC 20052, USA.

|October 30, 2023

概括

皮质 - 半神经过渡因素,如牛,在中枢神经系统 (CNS) 受伤后协调质反应. 调节牛影响微质激活,天体细胞反应性和皮层病变后的神经元存活.

科学领域:

神经科学是一个神经科学.
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

中枢神经系统 (CNS) 的损伤会触发质反应,包括微质激活和星球细胞反应.
控制这些质反应的精确细胞和分子机制尚不完全理解.

研究的目的:

调查表皮-半机体转录因子 (EMT-Fs) 在调解质对中枢神经系统损伤的反应中的作用.
阐明Snail的功能,一个EMT-F,在调节皮层损伤后的微质和天体细胞行为.

主要方法:

在动物模型中诱导成人皮层病变.
使用反感性寡核酸来对牛进行打击.
用于牛过度表达的微质向腺相关病毒 (AAV).
评估了微质激活,星细胞反应性 (包括C3表达和细胞),星细胞结合完整性,神经血管结构,神经细胞死亡和突触密度.

主要成果:

牛的 Knockdown 加剧了微质激活,天体细胞反应,神经元细胞死亡和突触损失.
牛的敲击导致了天体细胞结节和神经血管结构的破坏.
通过AAV过度表达牛改善了与伤害相关的几个特征.
观察到促炎性细胞因子表达的变化.

结论:

表皮 - 介质细胞过渡因子,特别是牛,在协调质对中枢神经系统损伤的反应方面发挥着至关重要的作用.
牛调节微质和天体细胞的行为,影响神经元生存和突触完整性等结果.
针对像Snail这样的EMT-F,可能为中枢神经系统受伤的恢复提供治疗策略.

关键词:

一个牛牛牛.神经炎症是一种神经炎症.反应性星球细胞的反应性星球细胞.有反应性的微质细胞.

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