多组学揭示了大多不同的转录和蛋白质水平对潮间的环境的反应
Lani U Gleason1, Florian J Fekete1, Richelle L Tanner2
1Department of Biological Sciences, California State University Sacramento, Sacramento, CA 95819, USA.
The Journal of experimental biology
|October 30, 2023
概括
生物体的应激反应显示出更多的仅转录或仅蛋白质的表达变化,而不是一致的变化. 蛋白质调节,而不仅仅是转录水平,决定了不同环境中的反应.
科学领域:
- 环境压力反应环境压力反应
- 分子生物学分子生物学
- 蛋白质组学和转录组学.
背景情况:
- 生物对环境压力的反应涉及复杂的转录和蛋白质水平机制.
- 基因表达和蛋白质丰富度之间的关系并不总是直接的.
研究的目的:
- 研究如何在不同的环境条件下转录组和蛋白组模式分离.
- 为了比较不同微生物息地和处理的贝的转录和蛋白质表达一致性.
主要方法:
- 分析了Mytilus californianus的配对转录和蛋白质组数据集.
- 从潮间的地点采集了贝样本,并接受了普通花园,低潮间和高潮间的外围植物治疗.
- 在数据集之间共享的1519个基因被评估为差异性表达模式.
主要成果:
- 无论是转录还是蛋白质表达,在全球范围内都区分了治疗方法,尽管存在许多差异.
- 差异表达发生在单独的转录 (1451) 或单独的蛋白质 (226) 中的频率比一致性 (68) 更高.
- 上调的皮相关转录与良性疾病有关;压力诱导的转录,但不是蛋白质,用于分子陪伴者.
结论:
- 转录水平与蛋白质水平调节的相对重要性因细胞功能和环境环境而异.
- 转录和蛋白质表达之间的一致性在良性和急性压力条件之间有所不同.
- 翻译诱导的值模型可以解释伴侣在压力下的表达模式.
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