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通过调节ZNF502/VIM轴,ENAH-202促进了口腔状细胞癌的癌症进展
Xinyue Zhang1,2, Xi Chen1,2, Dongyuan Sun1,2
1School of Stomatology, Weifang Medical University, Weifang, Shandong, China.
Cancer medicine
|October 30, 2023
概括
长非编码RNA ENAH-202 在口腔状细胞癌 (OSCC) 中被上调. 通过调节ZNF502/Vimentin轴,ENAH-202促进OSCC细胞的增殖和转移.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 口腔状细胞癌 (OSCC) 是一种普遍存在的恶性瘤.
- 长非编码RNAs (lncRNAs) 在OSCC发展中的作用是一个活跃的研究领域.
- 识别像lncRNAs这样的新型调节因子对于理解OSCC进展至关重要.
研究的目的:
- 研究lncRNA ENAH-202对口腔状细胞癌 (OSCC) 发展的调节作用.
- 阐明ENAH-202影响OSCC进展的潜在分子机制.
主要方法:
- 使用定量实时PCR (qPCR) 来检测OSCC组织和细胞系中的ENAH-202表达.
- 在体外和体内测试 (CCK-8,殖民地形成,transwell,异种移植,尾部静脉注射) 评估了ENAH-202的生物功能.
- RNA拉下,LS-MS/MS,RNA免疫沉降 (RIP) 和染色体免疫沉降 (ChIP) 试验确定了分子机制.
主要成果:
- 在OSCC组织和细胞中,ENAH-202被显著上调.
- ENAH-202在体外和体内促进了OSCC细胞的增殖,迁移和入侵.
- 通过与ZNF502结合,ENAH-202促进了维门 (VIM) 转录,影响了启用同类物 (ENAH) 和与上皮转介质过渡 (EMT) 相关的蛋白质.
结论:
- ENAH-202促进OSCC细胞的增殖和转移.
- 在OSCC进展中,ZNF502/Vimentin (VIM) 轴是ENAH-202调节的关键途径.
- ENAH-202在口腔状细胞癌的发展和进展中发挥着重要作用.
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