在 rhesus macaques (Macaca mulatta) 中估计淋巴细胞过率的新方法
Allison M Tomasino1,2, John D Olson1,2, George W Schaaf1,2
1Section on Comparative Medicine, Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, North Carolina.
Radiation research
|October 30, 2023
概括
施瓦茨公式准确地估计了 rhesus 的膜过率 (GFR),为辐射后评估损伤提供了更简单的方法. 这一发现有助于对这些动物的功能进行临床评估.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學.
- 放射学 放射学是一门学科.
- 比较医学是一种比较医学.
背景情况:
- 辐射的晚期影响包括慢性损伤 (CKI),影响发病率和死亡率.
- 淋巴膜过率 (GFR) 是功能的关键指标,但传统标记器可能无法检测早期损伤.
- 目前的GFR测量方法复杂且昂贵,需要更简单的替代方法.
研究的目的:
- 评估施瓦茨公式的适用性,通常用于人类儿科,用于估计 rhesus macaques 的 GFR.
- 为了确定施瓦茨公式估计的GFR (eGFR) 与被照射和未照射的iohexol测量GFR是否相关.
主要方法:
- 在12只具有不同血尿素 (BUN) 水平的 rhesus 和4个对照群中测量了iohexol 清除值.
- 使用注射后通过质谱测量确定的血IOHEXOL度来计算GFR.
- 施瓦茨公式被用来计算eGFR与iohexol-GFR进行比较.
主要成果:
- 在iohexol-GFR和Schwartz公式-eGFR之间观察到显著的正相关性.
- 在接受<6 Gy辐射的中,雄性比雌性呈现出更高的GFR.
- 与<6 Gy剂量相比,子在脏辐射剂量>6 Gy时显示出较低的GFR.
结论:
- 施瓦茨公式提供了一个快速的,非侵入性的,且具有成本效益的估计,对 rhesus macaques 的 GFR.
- 这种方法可以帮助临床评估功能和损伤在辐射.
- 这项研究验证了在非人类灵长类动物模型中对GFR估计的更简单方法.
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