在结性脊髓炎中,RUNX2基因促进体甲基化和转录水平的差异
Feier Wang1,2, Yuting Chen1,2, Jiangping Kong1,2
1Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, China.
International journal of rheumatic diseases
|October 30, 2023
概括
脊髓炎 (AS) 患者表现出RUNX2基因促进体的低甲基化,并增加RUNX2mRNA水平. 这些在RUNX2中的表观遗传和转录性变化可能有助于AS的发病.
科学领域:
- 免疫学 免疫学 免疫学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 化脊柱炎 (AS) 是一种使人虚弱的免疫疾病,影响患者的生活.
- 表观遗传修饰,特别是DNA甲基化,越来越被认为是复杂疾病的关键.
- 与Runt相关的转录因子2 (RUNX2) 在骨发育中起作用,并与各种疾病有关.
研究的目的:
- 为了研究AS患者RUNX2基因促进物甲基化变化的变化.
- 检查RUNX2促进体甲基化及其在AS中的转录水平之间的关系.
- 评估RUNX2甲基化和转录作为AS生物标志物的潜力.
主要方法:
- 量化实时逆转录聚合酶链反应 (qRT-PCR) 用于测量RUNX2mRNA水平.
- 对RUNX2基因促进体的DNA甲基化分析在AS患者和健康对照 (HCs) 中进行.
- 进行了相关性分析和接收器运行特征 (ROC) 曲线分析.
主要成果:
- 与HC患者相比,AS患者的RUNX2基因促进体显著低甲基化 (p < .001).
- 在AS患者中,RUNX2mRNA水平明显高于HC患者 (p < .05).
- RUNX2甲基化状态显示出作为生物标志物的潜力,以区分AS患者和HC患者.
结论:
- 在AS患者中,RUNX2基因促进体的异常低甲基化发生.
- 上调的RUNX2转录水平与AS中的促进物低甲基化相关.
- RUNX2表观遗传失调与结性脊柱炎的发病有关.
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