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来自双乳液模板微囊的尿素驱动微电机的不对称增强运动
Jessica Ann O'Callaghan1, Daeyeon Lee1, Daniel A Hammer1,2
1Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
ACS applied materials & interfaces
|October 30, 2023
概括
使用微流体工程设计的由酶驱动的微囊表现出自主运动. 表面的功能化和粗性显著提高了尿酶驱动的推进,为先进的原细胞工程和生物医学应用铺平了道路.
科学领域:
- 生物医学工程 生物医学工程
- 材料科学 材料科学 材料科学
- 化学工程是化学工程的重要组成部分.
背景情况:
- 酶驱动电机对于药物输送和细胞工程至关重要,但往往缺乏生物特征.
- 现有的系统通常依赖于泡推进,缺乏明确的边界.
研究的目的:
- 使用酶尿素酶,设计具有自主运动的细胞大小的微囊.
- 调查表面功能化和粗度对微囊推进的影响.
主要方法:
- 使用滴滴微流体制造多酸 (乳酸-合糖酸) 微囊.
- 使用聚乙醇 (PVA) 和聚乙烯-阿尔特-马莱克无水化物 (PEMA) 表面活性剂与尿素的功能化微囊表面.
- 通过结合二氧化纳米颗粒调整表面粗度.
主要成果:
- 聚乙烯-高氨酸无水化物 (PEMA) 在光滑的微囊上增加了尿素酶接种密度,使尿素能够运动.
- 带有PEMA的粗微囊表现出比光滑微囊快三倍的运动.
- 在粗的表面上不对称的尿酶分布可能导致了定向运动.
结论:
- 表面特性极大地影响微流体制备的微囊中的酶驱动运动.
- 这项工作为创造生物医学应用的运动原细胞提供了洞察力.
- 微流体是设计活跃微尺度系统的强大工具.
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