在芽和裂变酵母中量化流压力,基于透性质
1Department of Cell and Tissue Biology, University of California, San Francisco, CA 94143.
Molecular biology of the cell
|October 30, 2023
概括
测量像酵母这样的围墙细胞中的压是具有挑战性的. 这项研究引入了一种简单的基于原质细胞的方法来量化压,甚至在Saccharomyces cerevisiae菌株内也显示出显著的变化.
科学领域:
- 细胞生物物理学 细胞生物物理学
- 微生物学 微生物学
- 定量生物学的定量生物学.
背景情况:
- 围墙细胞,包括植物,真菌和细菌,保持高的内部水静压 (turgor压) 对于生长和形状至关重要.
- 精确测量压在实验上是很困难的,因为缺乏可靠的定量数据来测量芽酵母.
- 了解压对于细胞力学和进化研究至关重要.
研究的目的:
- 开发一种简单而强大的方法来量化酵母中的压.
- 为了比较不同酵母物种和菌株的压.
- 突出物种内部基本生物物理参数的变化.
主要方法:
- 使用酵母原生菌作为氧度计来确定同度.
- 采用了三种不同的方法来识别同位素条件: 3D 细胞体积,细胞质光体强度和纳米rheology 探针移动性.
- 确保在三种方法中保持一致,以可靠地估计压.
主要成果:
- 提供了 *Schizosaccharomyces pombe* (1.0 ± 0.1 MPa), *Schizosaccharomyces japonicus* (0.49 ± 0.01 MPa), *Saccharomyces cerevisiae* W303a (0.5 ± 0.1 MPa) 和 *Saccharomyces cerevisiae* BY4741 (0.31 ± 0.03 MPa) 的定量压值.
- 在*Saccharomyces cerevisiae*菌株之间观察到压和纳米rheology的显著差异.
- 在关键的生物物理参数中,即使在同一物种的野生型菌株中,也表现出了显著的变化.
结论:
- 开发的基于原塑的方法提供了一种可靠的方法来测量酵母压.
- 在酵母物种之间,甚至在*Saccharomyces cerevisiae**菌株内,压差异很大.
- 这些发现为细胞力学和比较进化生物学研究提供了重要的定量数据.
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