基于酸盐的可生物降解支架激活细胞能量代谢,加速伤口愈合
Min Wu1, Yitao Zhao1, Meihan Tao1
1Department of Histology and Embryology, GDMPA Key Laboratory of Key Technologies for Cosmetics Safety and Efficacy Evaluation, NMPA Key Laboratory for Safety Evaluation of Cosmetics, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, P. R. China.
ACS applied materials & interfaces
|October 30, 2023
概括
这项研究开发了新型的聚二醇 (酸) 支架,可以增强细胞能量生产. 这些生物能量支架通过促进细胞新陈代谢,促进组织再生和加速伤口愈合.
科学领域:
- 生物材料科学 生物材料科学
- 细胞生物能量学 细胞生物能量学
- 组织工程是组织工程.
背景情况:
- 细胞生物能学为组织再生提供了治疗潜力.
- 为组织修复开发具有长期生物能效的3D支架具有挑战性.
研究的目的:
- 研究l-酸盐在促进细胞能量代谢中的潜力.
- 制造和描述用于组织再生的新型生物能量多孔支架.
主要方法:
- 通过单多重凝结合成的聚二醇 (PDoM) 预聚合物.
- 通过热交联制造的多孔PDoM支架.
- 评估了脚手架降解产品对细胞代谢和ATP水平的影响.
- 评估了脚手架支持中细胞干细胞 (MSC) 生长和生物活性分子分泌的能力.
- 在大鼠全厚皮肤缺陷模型中测试了脚手架的有效性.
主要成果:
- PDoM支架的降解产品通过参与三碳酸 (TCA) 循环,增加腺三酸盐 (ATP) 水平来增强细胞能量代谢.
- 脚手架促进了细胞生物合成,包括 I 型原体,纤维蛋白 1,和actin alpha 2.
- PDoM支架支持MSC生长,并刺激了血管内皮生长因子 (VEGF),转化生长因子-β1 (TGF-β1) 和基本纤维细胞生长因子 (bFGF) 的分泌.
- 在大鼠模型中,载有干细胞的支架显著加速了伤口愈合.
结论:
- 开发的PDoM支架有效地促进细胞生物能和组织再生.
- 脚手架为再生医学中的治疗应用提供了一个有前途的平台.
- PDoM脚手架在创建用于伤口愈合的功能生物材料方面取得了重大进展.
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