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量化和调节蛋白质封装在基于关诺辛的超分子颗粒中的蛋白质封装

Luis A Prieto-Costas¹, Génesis R Rivera-Cordero¹, José M Rivera¹

¹Department of Chemistry and Molecular Sciences Research Center, University of Puerto Rico at Río Piedras, San Juan, Puerto Rico 00926, United States.

Bioconjugate chemistry

|October 30, 2023

概括

研究人员开发了一种流细胞测量方法,以量化超分子囊 (SHS) 颗粒中的蛋白质封装. 这种技术允许调整特定蛋白质结合的粒子特性,从而推进药物递送应用.

科学领域:

生物技术是生物技术.
材料科学材料科学材料科学
生物化学生物化学

背景情况:

蛋白质封装保存了结构和功能.
根据蛋白质特征量身定制保护剂可以调节特性.
超分子囊 (SHS) 粒子提供了一个新的封装平台.

研究的目的:

为量化SHS颗粒中的蛋白质封装适应流细胞计法.
评估SHS颗粒对于特定蛋白质结合的可调性.
为了研究粒子修饰对蛋白质亲和力的影响.

主要方法:

开发了一种流动细胞计量试验,以量化SHS颗粒中光素异硫酸 (FITC) 标记蛋白质的封装.
使用共聚焦激光扫描显微镜 (CLSM) 来成像蛋白质分布.
研究了阿维丁-生物素相互作用来调整SHS粒子亲和力.

主要成果:

量化蛋白质封装,显而易见的关联常数在微分子范围,用水性作为一个关键因素.
CLSM证实了颗粒中的蛋白质分布.
调整SHS颗粒的阿维丁与生物素的亲和力表明了和效应,可能是由于交叉链接.

结论:

流细胞计方法对于高通量,可重复的蛋白质封装量化是有效的.
SHS颗粒可以调整为特定的蛋白质结合,并有可能用于药物输送应用.
使用较低亲和度联体的进一步优化可以增强蛋白亲和度调制.

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