探索囊性纤维化中培养的鼻腔和支气管上皮质之间的内在变异性
Lisa W Rodenburg1,2, Mieke Metzemaekers3,4, Isabelle S van der Windt5,6
1Department of Pediatric Pulmonology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht University, Member of ERN-LUNG, 3584 EA, Utrecht, The Netherlands. l.w.rodenburg-2@umcutrecht.nl.
Scientific reports
|October 31, 2023
概括
来自囊性纤维化 (CF) 患者的鼻腔和支气管上皮细胞在细胞培养中保留了不同的特征. 细胞功能和基因表达的这些差异对于理解CF呼吸道疾病至关重要.
科学领域:
- 呼吸系统医学 呼吸系统医学
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 鼻腔和支气管上皮是受囊性纤维化 (CF) 影响的关键呼吸道组成部分.
- 鼻腔和支气管组织之间存在内在的变异性,包括细胞组成和基因表达.
- 胚胎层的起源影响了这些独特的细胞特性.
研究的目的:
- 为了调查鼻腔和支气管上皮细胞之间的内在差异是否在细胞培养中持续存在.
- 确定这些差异如何影响CF中的上皮细胞功能.
- 探索对理解CF病变发生的后果.
主要方法:
- 空气-液体界面 (ALI) 的差异化表皮细胞与CF受试者的比较.
- RNA测序以识别特定细胞类型的特征转录因子.
- ATAC测序用于评估前代细胞中的转录因子活性.
- 从鼻腔和支气管上皮细胞中获得的3D有机体的分析.
主要成果:
- 在鼻与支气管CF培养物中观察到明显的粘膜分化状态.
- 在分化细胞中确定了细胞类型特异的特征转录因子,其中一些在原始细胞中平衡.
- 鼻腔和支气管上皮质器官表现出不同的液体分泌能力,与状细胞分化有关.
结论:
- 鼻腔和支气管上皮细胞的独特表型和功能特征在细胞培养模型中持续存在.
- 这些持久的差异可以用来研究CF呼吸道疾病的组织特异性影响.
- 细胞培养模型为CF的上空和下空气道病理提供了有价值的见解.
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