可以通过调节自和亡途径来改善谷氨酸诱导的细胞损伤
Danni Xie1, Caiyou Song1, Tao Qin1
1State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China.
Scientific reports
|October 31, 2023
概括
传统中医药中的莫斯库斯 (Moschus) 显示出对谷氨酸诱导的细胞损伤的保护作用,这是阿尔茨海默氏症 (AD) 的一种模型. 它的机制涉及调节自和亡,这表明AD治疗的潜力.
科学领域:
- 神经科学是一个神经科学.
- 药理学 药理学是指药理学的学科.
- 传统中国医药 传统中国医药
背景情况:
- 阿尔茨海默病 (AD) 是一种神经退行性疾病,导致认知能力下降,治疗选择有限.
- 传统中国医学 (TCM),包括,正在研究对神经退行性疾病的潜在治疗效果.
- 马斯克对AD细胞模型的保护作用在很大程度上仍未被探索.
研究的目的:
- 为了研究马斯克对谷氨酸酸 (Glu) 诱导的PC12细胞损伤的保护作用,这是一个常见的阿尔茨海默氏症细胞模型.
- 预先探索莫斯库斯作用的潜在机制,重点关注自和亡途径.
主要方法:
- 用气色谱-质谱法 (GC-MS) 识别了莫斯科的化学化合物.
- PC12细胞暴露在谷氨酸中以诱导类似AD的细胞损伤模型.
- 分析了细胞活力,乳酸脱酶 (LDH) 释放,线粒体功能 (ROS,MMP),亡标记物和与自相关的蛋白质 (Beclin1,LC3II,BCL-2/BAX,p62).
主要成果:
- 暴露于谷氨酸导致细胞活力下降,LDH释放增加,线粒体损伤,亡和改变的自标志物.
- 使用莫斯克的预处理显著减弱了谷氨酸诱导的细胞损伤和亡.
- 莫斯克调节了与亡相关的蛋白质 (切割caspase-3,Bcl-2/BAX) 和与自相关的蛋白质 (Beclin1,LC3II,p62) 的表达.
结论:
- 蚊子表现出对谷氨酸诱导的PC12细胞损伤的保护性活性.
- 蚊子的保护机制可能涉及细胞自和亡途径的调节.
- 树有潜力作为阿尔茨海默氏症和其他神经退行性疾病的治疗药物.
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