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相关概念视频

CRISPR01:59

CRISPR

52.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Homologous Recombination02:31

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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相关实验视频

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Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
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使用CRISPR/基于Cas的基因组编辑方法的干细胞的最新研究趋势

Da Eun Yoon1,2, Hyunji Lee1,3, Kyoungmi Kim1,2

  • 1Department of Biomedical Sciences, Korea University College of Medicine, Seoul, Korea.

International journal of stem cells
|October 31, 2023
PubMed
概括

克里斯普尔基因编辑技术能够在生物体,包括干细胞中精确地改变DNA. 本综述探讨了CRISPR/Cas在干细胞研究中的应用,以了解疾病和新的治疗策略.

关键词:
聚类有规律间隔的短palindromic重复.基因组编辑 基因组编辑干细胞是一种干细胞.转录调节器的调节器

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科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 干细胞研究 干细胞研究

背景情况:

  • 集群定期间隔的简短的Palindromic重复 (CRISPR) 系统是一种强大的基因组编辑技术.
  • 克里斯普尔允许精确的DNA修改,包括单核酸转换,基因敲进,染色体重排和基因破坏.
  • 基于CRISPR的表观遗传调节可以在不引起DNA损伤的情况下实现.

研究的目的:

  • 审查使用CRISPR/Cas技术的干细胞研究的最新进展.
  • 讨论CRISPR介导的干细胞工程在了解疾病发病的潜力.
  • 探索CRISPR应用在治疗不可治愈疾病中的未来前景.

主要方法:

  • 审查目前用于干细胞研究的CRISPR/Cas技术.
  • 对基因编辑能力进行分析,以准确地改变DNA.
  • 使用CRISPR系统检查表观遗传调节策略.

主要成果:

  • 克里斯普技术促进了干细胞中多样化和精确的基因组编辑.
  • 克里斯普尔应用程序提供了对疾病机制和潜在治疗点的见解.
  • 目前正在探索CRISPR-Cas系统对干细胞的表观遗传修饰.

结论:

  • 在干细胞工程方面,CRISPR/Cas技术具有显著的前景.
  • 干细胞基因编辑对于推进疾病研究和开发治疗方法至关重要.
  • 在干细胞治疗中,CRISPR的未来应用对于各种疾病是广泛的.