快速而灵敏的LC-MS/MS方法用于量化大麻素及其代谢物在养大麻的牛血中的含量
1Pennsylvania Animal Diagnostic Laboratory System Toxicology Laboratory, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, Pennsylvania, USA.
Journal of separation science
|October 31, 2023
概括
一种新的液体色谱-并联质谱法准确量化牛血中的大麻素. 发现的主要代谢物是7-碳酸大麻二醇,在大麻消费后几周内可以检测到.
科学领域:
- 农业科学 农业科学
- 分析化学 分析化学
- 兽医医学 兽医医学 兽医医学
背景情况:
- 大麻作为牲畜料,由于潜在的大麻素转移,引发了安全问题.
- 准确的检测和量化方法对于评估动物中的大麻素水平至关重要.
- 了解牲畜中的大麻素代谢对于料安全法规至关重要.
研究的目的:
- 开发和验证一种快速,灵敏的方法来量化牛血中的大麻素及其代谢物.
- 应用该方法来评估牛的麻基材料中大麻素的存在.
- 为了确定牛血中占主导地位的大麻素及其代谢物.
主要方法:
- 液体染色学-并联质谱学 (LC-MS/MS) 用于量化.
- 使用了一种联合的清洁程序,包括Captiva增强矩阵去除-脂质和出辅助液体-液体提取.
- 方法验证评估了准确性,精度,特异性和灵敏度.
主要成果:
- 开发的LC-MS/MS方法表现出高灵敏度,准确度和精度.
- 在牛血中检测到各种大麻素,包括delta-9-tetrahydrocannabinol.
- 作为主要化合物,鉴定出一种大麻代谢产物7-碳氧大麻.
结论:
- 建立了一种可靠的LC-MS/MS方法来分析牛血中的大麻素.
- 食大麻导致牛体内可检测到的大麻素水平和特定的代谢物.
- 7碳酸在血中持续存在,这表明大麻消费后的长期检测能力.
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