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通过调节CCNA1/CREBBP转录,RNF6促进胃癌的进展

Qiuyu Jiang¹, Jiyu Miao², Fei Wu³

¹Department of Cell Biology and Genetics/Key Laboratory of Environment and Genes Related to Diseases, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.

Cell cycle (Georgetown, Tex.)

|October 31, 2023

概括

环指蛋白6 (RNF6) 在胃癌 (GC) 细胞的核中起作用,而不是作为E3泛基因酶. 通过调节CCNA1和CREBBP基因转录,RNF6促进了GC的进展.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
基因法规基因法规

背景情况:

环指蛋白6 (RNF6) 被确定为一种E3无素联酶,与胃癌 (GC) 的进展有关.
以前的研究表明,RNF6在GC中扮演着泛素酶的角色,但其确切的功能仍然不完全理解.

研究的目的:

为了研究RNF6在胃癌中的非-ubiquiquitin结合酶功能.
阐明RNF6影响GC细胞生物功能的机制.

主要方法:

染色体免疫沉降测序 (ChIP-seq) 用于识别RNF6的DNA结合部位.
分析RNF6对基因表达 (CCNA1,CREBBP) 和GC细胞功能的影响.
基因沉默实验以验证CCNA1和CREBBP的作用.

主要成果:

RNF6在GC细胞中表现出明确的核定位,这表明其作用超出了E3泛基因酶活性.
ChIP-seq揭示了RNF6与DNA结合,并调节参与细胞循环和亡的基因.
RNF6直接与循环A1 (CCNA1) 和CREB结合蛋白 (CREBBP) 的促进子区域结合,调节它们在GC中的转录.
沉默CCNA1/CREBBP部分扭转了RNF6过度表达的前瘤性影响.

结论:

通过对CCNA1和CREBBP的转录调节,RNF6促进胃癌的进展.
RNF6在GC中充当核转录因子,与其先前已知的E3泛基因酶功能不同.
准RNF6-CCNA1/CREBBP轴可能为胃癌提供一种新的治疗策略.

关键词:

CCNA1 CCNA1 的意思是 CCNA1 的意思.这就是CREBBP.在 RNF6 中.胃癌胃癌是一种胃癌.转录规则转录规则转录规则

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