相关实验视频

Updated: Jul 12, 2025

Generation of a Chronic Obstructive Pulmonary Disease Model in Mice by Repeated Ozone Exposure

Generation of a Chronic Obstructive Pulmonary Disease Model in Mice by Repeated Ozone Exposure

Published on: August 25, 2017

KLF9在慢性阻塞性肺部呼吸道炎症中的机制

Peijie Gu¹, Zhen Wang¹, Xin Yu¹

¹Department of Pulmonary and Critical Care Medicine, Jiangyin Hospital of Traditional Chinese Medicine, Jiangyin City, China.

Immunity, inflammation and disease

|October 31, 2023

概括

克鲁佩尔类因子9 (KLF9) 通过调节miR-494-3p和PTEN促进慢性阻塞性肺病 (COPD) 的呼吸道炎症. 抑制KLF9可缓解COPD中的炎症和NLRP3炎症酶激活.

科学领域:

肺部医学肺部医学
分子生物学分子生物学
炎症研究炎症研究

背景情况:

慢性阻塞性肺病 (COPD) 的特点是慢性呼吸道炎症.
了解推动COPD病变的分子机制对于开发有效的治疗方法至关重要.

研究的目的:

调查克鲁佩尔类因子9 (KLF9) /微RNA (miR) -494-3p /酸酶和张素同位素 (PTEN) 轴在COPD相关的呼吸道炎症中的作用.
建立针对这一轴的新型COPD治疗策略的理论基础.

主要方法:

已建立的COPD小鼠和人类肺上皮细胞模型使用香烟烟雾暴露.
量化炎症标志物,细胞活力和KLF9,miR-494-3p,PTEN和NLRP3的表达水平.
利用染色体免疫沉和双化酶试验来确认分子相互作用.

主要成果:

在COPD肺组织中,KLF9被显著上调.
抑制KLF9可以减少呼吸道炎症,炎症细胞透和NLRP3的表达.
KLF9促进了miR-494-3p的表达,这反过来又对PTEN进行了负面调节.
miR-494-3p的过度表达抵消了KLF9对NLRP3炎症和炎症的淘汰效应.

结论:

KLF9通过调节miR-494-3p并随后抑制PTEN来促进NLRP3炎症酶介导的COPD炎症.
准KLF9/miR-494-3p/PTEN轴为COPD提供了一个潜在的治疗途径.

关键词:

在KLF9KLF9KLF9 在NLRP3中,NLRP3是NLRP3中的一个.在这里,我们可以使用PTEN.呼吸道炎症呼吸道炎症支气管支气管洗液的支气管支气管慢性阻塞性肺病慢性阻塞性肺病香烟烟雾提取物香烟烟雾提取物在 miR-494-3p 中使用.

更多相关视频

The Utilization of Oropharyngeal Intratracheal PAMP Administration and Bronchoalveolar Lavage to Evaluate the Host Immune Response in Mice

The Utilization of Oropharyngeal Intratracheal PAMP Administration and Bronchoalveolar Lavage to Evaluate the Host Immune Response in Mice

Published on: April 2, 2014

Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy

Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy

Published on: April 16, 2019

相关概念视频

Chronic Obstructive Pulmonary Disease-II: Pathophysiology

Chronic Obstructive Pulmonary Disease-II: Pathophysiology

Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation

Chronic Obstructive Pulmonary Disease-I: Introduction

Chronic Obstructive Pulmonary Disease-I: Introduction

Chronic Obstructive Pulmonary Disease (COPD) is a long-lasting respiratory condition requiring continuous attention and care. It is a progressive lung disease that leads to breathing challenges due to airflow obstruction. It manifests as persistent respiratory symptoms and restricted airflow resulting from abnormalities in the airways and alveoli, usually due to long-term exposure to harmful particles or gases. COPD mainly consists of two primary conditions: emphysema and chronic bronchitis.

Chronic Obstructive Pulmonary Disease

Chronic Obstructive Pulmonary Disease

COPD is defined as a heterogeneous lung condition marked by persistent respiratory symptoms such as dyspnea, cough, and sputum production, caused by abnormalities in the airways that cause airflow obstruction.
Smoking is a primary risk factor for COPD, with over 80% of patients having a history of it. Patients typically experience progressive dyspnea or labored breathing, frequent coughing, and recurrent pulmonary infections. Many eventually succumb to respiratory failure, characterized by...

Asthma-II: Pathophysiology and Classification

Asthma-II: Pathophysiology and Classification

Asthma is a prevalent chronic respiratory condition marked by inflammation and hyperresponsiveness of the airways. Its pathophysiology involves complex interactions among inflammatory pathways, immune responses, and neural mechanisms.
Additionally, environmental and genetic factors play crucial roles in determining an individual's susceptibility to asthma and the severity of their condition.
Critical processes in asthma pathophysiology include:

COPD: Pathogenesis and Clinical Features

COPD: Pathogenesis and Clinical Features

Chronic obstructive pulmonary disease (COPD) is a group of lung conditions that progressively worsen over time, including chronic bronchitis and emphysema. This cluster of diseases collectively leads to a gradual and irreversible decline in lung function over time.
The primary cause for the onset of COPD is cigarette smoking and exposure to air pollution. These hazardous factors initiate a chain reaction within the lungs, resulting in chronic inflammation, damage to the airways, and a...

Chronic Obstructive Pulmonary Disease-III: Symptoms and Complications.

Chronic Obstructive Pulmonary Disease-III: Symptoms and Complications.

Understanding the variety of primary symptoms and systemic complications that characterize chronic obstructive pulmonary disease (COPD) is crucial for healthcare professionals.
Symptoms of COPD can be classified as primary or systemic. Primary symptoms relate to reduced airflow, while systemic or extrapulmonary symptoms relate to COPD's broader impact on the body.
Primary Symptoms of COPD: