Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

The Nucleosome Core Particle02:10

The Nucleosome Core Particle

12.1K
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
12.1K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

23.3K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
23.3K
Position-effect Variegation02:32

Position-effect Variegation

6.3K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
6.3K
Nucleosome Remodeling02:54

Nucleosome Remodeling

9.2K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
9.2K
The Nucleosome02:33

The Nucleosome

16.3K
DNA in a human cell is almost 2m long and it is packed inside a tiny nucleus that is only a few microns in diameter. The level of compaction of DNA inside the nucleus is astonishing. It is organized into several sequentially higher levels of compaction to fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
DNA is wound twice around a protein complex called histone core, that consist of 8 histone proteins. This complex...
16.3K
Chromatin Packaging02:21

Chromatin Packaging

15.4K
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order...
15.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Advances in Antibiotic Heteroresistance in Acinetobacter baumannii.

Biochimie·2026
Same author

Prediction of the Compressive Strength of Tailings-Based Cement Material Using Machine Learning Models with Experimental Validation.

Materials (Basel, Switzerland)·2026
Same author

CCR4-NOT is required for suppressing pervasive transcription and retrotransposable elements.

The Journal of biological chemistry·2026
Same author

Oncofetal RNA-binding proteins of IGF2BP family suppress IRF-3 and NF-kB dependent transcription downstream of cytosolic RNA sensors.

bioRxiv : the preprint server for biology·2026
Same author

OSBPL10 Drives Lipophagy-Mediated Lipid Mobilization to Promote Pancreatic Ductal Adenocarcinoma Progression.

International journal of biological sciences·2026
Same author

ITS-ShipFormer: An Informative Token Selection Former for SAR Ship Recognition.

IEEE transactions on neural networks and learning systems·2026

相关实验视频

Updated: Jul 12, 2025

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis
10:05

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

Published on: December 12, 2017

22.2K

SEM:基于大小的预期最大化,用于表征核位和亚型.

Jianyu Yang1, Kuangyu Yen2,3, Shaun Mahony1

  • 1Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, USA.

bioRxiv : the preprint server for biology
|October 31, 2023
PubMed
概括

一个新的工具,基于尺寸的预期最大化 (SEM),从MNase-seq数据中识别出不同的核细胞类型. SEM在调节区域中揭示了短片核体,有助于研究非典型核体及其功能.

科学领域:

  • * 分子生物学 * 分子生物学
  • * 基因组学 是一个学科.

更多相关视频

A Novel Bayesian Change-point Algorithm for Genome-wide Analysis of Diverse ChIPseq Data Types
12:39

A Novel Bayesian Change-point Algorithm for Genome-wide Analysis of Diverse ChIPseq Data Types

Published on: December 10, 2012

11.4K
A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies
08:04

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies

Published on: August 13, 2020

3.5K

相关实验视频

Last Updated: Jul 12, 2025

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis
10:05

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

Published on: December 12, 2017

22.2K
A Novel Bayesian Change-point Algorithm for Genome-wide Analysis of Diverse ChIPseq Data Types
12:39

A Novel Bayesian Change-point Algorithm for Genome-wide Analysis of Diverse ChIPseq Data Types

Published on: December 10, 2012

11.4K
A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies
08:04

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies

Published on: August 13, 2020

3.5K
  • * 表观遗传学 是一种表观遗传学.

    • 背景情况:

    • * 核形状通常使用MNase-seq,假设统一的147bp DNA保护.
    • *现有的分析工具忽略了较短的DNA片段,可能缺失了非典型核细胞的调节作用.
    • *核细胞DNA保护长度的变化与基因组组成和化学修饰有关.

    结论:

    • * SEM有效地从MNase-seq数据中区分不同的核细胞亚型.
    • *这种能力可以更深入地研究非标准核细胞的功能作用.
    • *这些发现强调了在基因组研究中考虑核细胞异质性的重要性.