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相关概念视频

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Updated: Jul 12, 2025

Author Spotlight: Importance of Single Cell Sorting in Isolating Purified Populations of Mesenchymal Stem Cells
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通过刺激的拉曼激活细胞喷射来进行高通量单细胞分类.

Jing Zhang1,2, Haonan Lin1,2, Jiabao Xu3

  • 1Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA.

bioRxiv : the preprint server for biology
|October 31, 2023
PubMed
概括
此摘要是机器生成的。

一个新的刺激拉曼激活细胞喷射 (S-RACE) 平台使用化学成像实现高通量单细胞分类. 这种方法可以为生物学和医学中的应用提供精确的细胞隔离.

关键词:
生物科学 生物科学生物物理和计算生物学拉曼光谱法 拉曼光谱法 拉曼光谱法细胞喷射细胞的喷射.细胞分类 细胞分类高通量分析的分析方法激光诱导的前向转移是激光诱导的.单个微生物的隔离和培养

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科学领域:

  • 生物技术是生物技术.
  • 化学成像技术 化学成像技术
  • 细胞生物学 细胞生物学

背景情况:

  • 单细胞分类对于理解细胞异质性至关重要.
  • 拉曼激活细胞分类 (RACS) 提供无标签分类,但面临吞吐量限制.
  • 自发拉曼散射的截面小,限制了当前的RACS速度.

研究的目的:

  • 开发一个高通量单细胞分类平台.
  • 为了克服现有的RACS方法的吞吐量限制.
  • 为了实现精确的基于化学的细胞分类和分析.

主要方法:

  • 利用刺激拉曼散射用于高分辨率化学成像.
  • 集成的现场图像分解和激光诱导的细胞喷射.
  • 开发了用于实时分类的闭环反控制.

主要成果:

  • 实现了95%的产量,98%的纯度和14事件/秒的吞吐量分类聚合物珠.
  • 根据化学配置文件,成功地从Saccharomyces cerevisiae中分类了富含脂质的Rhodotorula glutinis.
  • 从老鼠大脑组织中实时分类和弹出特定区域.

结论:

  • 该S-RACE平台提供高通量,无标签的单细胞分类.
  • 能够进行活细胞分类,用于随后培养细菌和真菌.
  • 在各种生物和医学领域为单细胞应用开辟了新的途径.