孕激素通过两个具有PLA2活性的有约束性共受体诱导半变异
Nancy Nader1,2, Lama Assaf1,3, Lubna Zarif1
1Calcium Signaling Group, Research Department, Weill Cornell Medicine Qatar, Education City, Qatar Foundation, Doha, Qatar.
bioRxiv : the preprint server for biology
|October 31, 2023
概括
孕激素 (P4) 通过膜受体 (mPRs) 快速触发卵细胞成熟. 这项研究确定了ABHD2作为具有脂酶A2活性的共同受体,启动了减量过程中必不可少的信号级联.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 细胞信号传递 细胞信号传递
背景情况:
- 孕激素 (P4) 是一种调节生殖和代谢功能的类固醇激素.
- 传统上,P4通过核受体发出信号,但也通过快速的,涉及膜受体 (mPRs) 的非基因组途径发出信号.
- 非基因组P4信号传输的精确信号传导级联仍然在很大程度上是未知的.
研究的目的:
- 阐明了快速,非基因的孕激素信号的基础上的分子机制.
- 识别信号转导途径的关键组成部分,触发卵细胞成熟.
- 为了研究ABHD2在孕激素介导的半衰期中的作用.
主要方法:
- 利用Xenopus卵细胞成熟作为研究非基因组P4信号的模型系统.
- 采用功能测定和无偏见/有针对性的基于细胞的脂质组学.
- 研究了ABHD2与mPRβ结合的脂酶A2 (PLA2) 活性.
主要成果:
- 鉴定了脂质酸酶ABHD2作为与mPRβ一起的必不可少的共受体,用于触发微变.
- 证明ABHD2具有mPRβ依赖的PLA2活性.
- 表明P4信号诱导ABHD2 PLA2活性,导致mPRβ内细胞分裂和G蛋白结合受体激活剂的产生.
结论:
- 孕激素通过一种新的非基因组途径驱动卵细胞成熟.
- 这种途径需要mPRβ和ABHD2作为义务共受体.
- 由P4和mPRβ刺激的ABHD2的PLA2活性会产生脂质信使,这些信使会传播半分裂的信号.
相关概念视频
Meiosis II
183.5K
Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each...
183.5K
Meiosis I
40.8K
Meiosis is the division of a diploid cell into haploid cells forming sperm and eggs in animals through differentiation. Meiosis I is the first stage of meiosis, where the genetic recombination of homologous chromosomes and the reduction of the ploidy level by half occurs.
Prophase I is the most extended and complex step of meiosis I characterized by synapsis, chromosome pairing, and recombination of the homologous chromosomes. This process is facilitated by a proteinaceous structure called the...
Prophase I is the most extended and complex step of meiosis I characterized by synapsis, chromosome pairing, and recombination of the homologous chromosomes. This process is facilitated by a proteinaceous structure called the...
40.8K
Gonadal and Placental Hormones
1.5K
The gonads, namely the testes in males and the ovaries in females, are pivotal in producing gonadal hormones that orchestrate the intricate processes of sexual development and reproduction.
In males, testosterone is the primary gonadal androgen. It plays a central role in the maturation of male reproductive organs — the penis and testes. Additionally, testosterone is instrumental in the development of secondary sexual characteristics — a deep voice as well as facial and pubic hair...
In males, testosterone is the primary gonadal androgen. It plays a central role in the maturation of male reproductive organs — the penis and testes. Additionally, testosterone is instrumental in the development of secondary sexual characteristics — a deep voice as well as facial and pubic hair...
1.5K
Meiosis vs. Mitosis
56.8K
Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
56.8K
Co-activators and Co-repressors
7.4K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.4K
Positive Regulator Molecules
106.2K
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
106.2K


