在模仿老化骨髓的工程骨类型中,增加的变形是细胞机制反应的必需品
Alexander M Regner1, Maximilien DeLeon2,3,4, Kalin D Gibbons1
1Mechanical and Biomedical Engineering Department, Boise State University.
bioRxiv : the preprint server for biology
|October 31, 2023
概括
低强度振动 (LIV) 在老年骨模型中没有改善介质干细胞 (MSC) 反应. 骨体积分数,而不是压力,决定了MSC的行为,突出显示了机械传导需要可访问的骨表面.
科学领域:
- 生物医学工程 生物医学工程
- 机械生物学 机械生物学
- 干细胞生物学 干细胞生物学
背景情况:
- 衰老和太空飞行导致骨质流失,影响介质干细胞 (MSC) 和它们对机械刺激的反应.
- 骨髓中机械信号的减少可能会导致与年龄相关的骨质损失.
- 低强度振动 (LIV) 在老化和微重力模型中显示出恢复MSC功能的潜力.
研究的目的:
- 调查骨体积分数和机械应变在MSC对LIV的反应中的作用.
- 开发和验证用于研究骨机械生物学的3D骨髓模拟.
- 为了确定是否减少机械信号传递有助于老年人骨机械反应的下降.
主要方法:
- 使用聚乳酸 (PLA) 开发具有受控的脊髓几何和力学的3D骨髓模拟.
- 有限元 (FE) 建模,在LIV期间量化水凝内的应变分布.
- 封装MSCs在水凝在打印的trabeculae和评估LIV治疗后的细胞行为.
主要成果:
- 在MSC中,LIV治疗增加了I型原蛋白和F-actin.
- FE模型预测骨体积分数较低 (13%的BV/TV) 的类似物与较高 (25%的BV/TV) 的类似物相比,具有更高的菌株.
- 尽管菌株较高,但低骨体积分数类型的MSC显示原I和F-actin减少,表明骨表面可用性至关重要.
结论:
- 对LIV的MSC反应独立于水凝菌株,并受骨体积分数 (可用的骨表面) 的直接影响.
- 骨髓类似物为研究骨机械生物学提供了一个可靠的平台.
- 研究结果表明,优化骨表面可用性可能对维持MSC功能和骨健康至关重要.
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