对细菌的影响:通过多omics方法从大肠杆菌的见解
Martin Rihacek1, Ludmila Kosaristanova1, Tatiana Fialova1
1Department of Chemistry and Biochemistry, Faculty of AgriSciences, Mendel University in Brno, Brno, Czechia.
mSystems
|October 31, 2023
概括
长期接触氧化和纳米颗粒会导致细菌产生多药耐药性和毒性. 虽然有些变化是逆转的,但基因组变化仍然存在,通过料补充剂影响动物和人类的健康.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 毒理学 毒理学 毒理学
背景情况:
- 氧化 (ZnO) 和ZnO纳米颗粒广泛用于动物料补充剂.
- 长期暴露于这些化合物可以显著改变细菌种群.
- 肠道微生物群的改变可能对动物和人类健康构成风险.
研究的目的:
- 研究长期氧化物和氧化物纳米粒子暴露对细菌形态和生理学的影响.
- 为了确定细菌表型是否在去除后恢复到原来的状态.
- 识别基因组,转录基因组和蛋白质基因组级别的持续变化.
主要方法:
- 细菌培养对氧化和氧化纳米颗粒的暴露.
- 监测形态和生理变化.
- 暴露后和去除后对基因组,转录组和蛋白质组资料的分析.
主要成果:
- 观察到细菌形态和生理学的显著变化.
- 发现了多种药物耐药性和增强的毒性特征的出现.
- 虽然发生了表型逆转,但持续的基因组,转录组和蛋白质组变化仍然存在.
结论:
- 长期暴露会诱导细菌的适应性变化,包括耐药性和毒性.
- 分子变化的持久性突出了潜在的长期生态和健康影响.
- 监测料补充剂对于减轻与改变肠道微生物群相关的风险至关重要.
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