在微流体平台上并行检测化学反应,使用超极化核磁共振
Jose Yeste1, Marc Azagra1, Maria A Ortega1
1Institute for Bioengineering of Catalonia, The Barcelona Institute of Science and Technology, Barcelona, Spain. imarco@ibecbarcelona.eu.
Lab on a chip
|October 31, 2023
概括
溶解动态核极化 (dDNP) 与微流体学相结合,可实现化学反应的高通量实时分析. 这种先进的技术克服了以前的局限性,允许同时测量具有增强NMR灵敏度的多个反应.
科学领域:
- 核磁共振 (NMR) 光谱学 核磁共振 (NMR) 光谱学
- 化学反应分析 化学反应分析
- 微流体学 微流体学
背景情况:
- 溶解动态核极化 (dDNP) 显著提高了NMR的灵敏度,使实时反应监测成为可能.
- 对于反应分析的dDNP的局限性包括缓慢的极化积累和快速的信号衰减,阻碍了实验吞吐量.
- 目前的方法缺乏同时对多种化学反应进行高吞吐量分析的能力.
研究的目的:
- 开发一种微流体装置,用于使用dDNP-MR光谱成像对化学反应进行高通量现场分析.
- 为了克服与dDNP相关的低实验吞吐量和快速信号衰减的局限性.
- 为了使在相同的超极化条件下同时测量多个反应.
主要方法:
- 开发一种与dDNP-MR光谱成像相容的微流体装置.
- 使用单个dDNP样本同时测量最多8种化学反应.
- 使用通过过氧化脱碳化pyruvate的概念证明演示.
主要成果:
- 微流体dDNP-MR系统允许在多种化学反应中同时检测反应物和产物.
- 在一致的超极化条件下 (极化,度,pH,温度) 生成多个MR光谱数据集.
- 在pyruvate脱碳化中成功确定了反应物,包括2-hydroperoxy-2-hydroxypropanoate, peroxymonocarbonate 和 CO2.
结论:
- 与微流体学相结合的dDNP-MR光谱成像为分析化学反应中的多个组件提供了一种新的高通量方法.
- 这种方法有助于追踪以前无法通过标准MR检测到的快速化学反应.
- 该技术有望用于研究应用的生物样本中超极化基质的非破坏性现场代谢分析.
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