小麦bZIPC1与FT2相互作用,并有助于调节每个尖的尖数量
Priscilla Glenn1, Daniel P Woods1,2, Junli Zhang1
1Department of Plant Sciences, University of California, Davis, CA, 95616, USA.
概括
小麦转录因子bZIPC1与FT2相互作用,影响尖和粒数. 一个天然的bZIPC1等位基因 (H1) 显著增强了这些特征,帮助小麦育种提高产量.
科学领域:
- 植物分子生物学 植物分子生物学
- 小麦遗传学 小麦遗传学
- 提高作物质量 提高作物质量
背景情况:
- 在花T2 (FT2) 中的功能丧失突变会影响每的小麦尖子数量 (SNS).
- FT2与特定的转录因子相互作用,但不是A组bZIPs.
- 了解FT2相互作用对于提高小麦产量至关重要.
研究的目的:
- 确定与小麦FT2.2相互作用的蛋白质.
- 研究bZIPC1在小麦发育和产量特征中的作用.
- 在繁殖应用中对bZIPC1的自然变异进行表征.
主要方法:
- 使用FT2作为诱的酵母二混合选.
- 对bZIPC1和FT2基因表达模式的分析.
- 在bZIPC-A1和bZIPC-B1.1中产生联合功能丧失突变.
- 在bZIPC-B1基因区域的自然变异的哈普洛型分析.
主要成果:
- 鉴定了bZIPC1,一种特定于草的C组bZIP转录因子,与FT2相互作用.
- 结合的bzipc1功能丧失突变大大减少了四平状小麦中的SNS.
- 在bZIPC-B1中发现了三个主要的单元类型 (H1-H3),其中H1显著增加了SNS,粒数和粒重.
- 在现代小麦品种中,有利的H1单元型 (bZIPC-B1b等位基因) 更常见.
结论:
- bZIPC1与FT2相互作用,在调节小麦和谷粒数量方面发挥关键作用.
- 一个天然的bZIPC1等位基因 (H1) 为提高小麦产量提供了重要的潜力.
- 对有利的bZIPC1等位基因的诊断标记可以加速面食和面包小麦的育种计划.
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