建立一个高效的的短暂转化系统,以维蒂斯维尼费拉cv. "沙多内"是"沙多内"的意思
Jieping Wu1,2,3, Junxia Zhang1,2,3, Xinyi Hao1,2,3
1College of Enology and Horticulture, Ningxia University, Yinchuan, 750021, Ningxia, China.
Protoplasma
|October 31, 2023
概括
研究人员开发了一种快速的Agrobacterium介导的短暂转化系统,用于葡萄的calli. 这种方法增强了基因功能识别,通过过度表达VaCIPK18基因,提高了葡萄的耐寒性.
科学领域:
- 植物生物技术 植物生物技术
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- 葡萄 (Vitis L.) 的基因转换具有挑战性,阻碍了基因功能研究.
- 一种快速有效的葡萄转化方法对于作物改善至关重要.
研究的目的:
- 建立一个精简的Agrobacterium介导的短暂转化系统,用于葡萄卡利.
- 评估该系统对基因功能分析的有效性,特别是对于耐寒性.
主要方法:
- 优化的诱导和扩散介质 (B5介质具有特定的植物生长调节剂).
- 使用超声波,真空透和共同培养的Agrobacterium tumefaciens EHA105介导的转化.
- VaCIPK18基因的短暂转变以及基因表达和寒冷耐受性的评估.
主要成果:
- 确定了最佳的转化条件,实现高GUS酶活性.
- 在葡萄中成功的VaCIPK18基因的短暂表达.
- 与对照组相比,VaCIPK18过度表达的calli表现出明显改善的寒冷耐受性.
结论:
- 已经开发出一种简单而有效的葡萄的过渡转化系统.
- 这个系统有助于快速评估葡萄中的基因功能.
- VaCIPK18基因增强葡萄的寒冷耐受性,提供了提高作物的潜力.
相关概念视频
Plant Tissue Culture
38.0K
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
38.0K
Transgenic Plants
7.3K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.3K
In-vitro Mutagenesis
14.0K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
14.0K
Bacterial Transformation
55.4K
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
55.4K


