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构建基于库马林的生物直角宏分子探针用于光激活

Shuke Yang¹, Liang-Hua Zou¹, Runqi Li¹

¹School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, China.

ACS applied materials & interfaces

|October 31, 2023

概括

研究人员开发了用于生物成像的新型可光激活光探头 (K1-K4). 探测器K3显示聚合诱导的排放,而K3/K4使细胞内蛋白质的长期跟踪成为可能.

科学领域:

化学生物学化学生物学
分子成像学分子成像学
生物技术是生物技术.

背景情况:

可光激活光成像对于研究蛋白质动力学至关重要.
现有的探测器在灵敏度和长期跟踪方面存在局限性.
生物直角探针可以精确控制光激活.

研究的目的:

设计和合成新的可光激活的生物直角光探针.
为了评估它们在体外和活细胞中的性能.
建立一个平台,用于先进的蛋白质成像.

主要方法:

合成了四个基于库马林的光探针 (K1-K4) 带有光活性和HaloTag连接体.
光刺激反应和聚合诱导辐射 (AIE) 的表征.
探针与HaloTag融合感兴趣的蛋白质 (POI) 结合,并评估光成像能力.

主要成果:

探头K1-K4显示出快速的光刺激反应;K3显示出AIE.
结合探头 (K_POI) 在光激活时显示光增强.
K3_GFP_Halo和K4_GFP_Halo显示FRET,使蛋白质复合物的可视化成为可能.
K3和K4促进了IRE1_Halo蛋白的长期细胞内追踪.

结论:

建立了一个新的生物直角光探针平台,用于HaloTag融合蛋白质.
这些探头允许在体外和细胞环境中进行快速光激活.
开发的探测器增强了可视化蛋白质定位,贩运和相互作用的能力.

关键词:

在HaloTag中使用HaloTag.生物对等的结合关系.库马林是一种素.光成像成像技术的使用.摄影激活的使用.

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