相关实验视频

Updated: Jul 12, 2025

Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay

Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay

Published on: November 25, 2022

乙醇通过抑制ROS信号来抑制大米种子的发芽

Jiameng Chen¹, Zeyan Jin¹, Longyi Xiang¹

¹Department of Agronomy, Zijingang Campus, Zhejiang University, Hangzhou, 310058, PR China.

Journal of plant physiology

|October 31, 2023

概括

乙醇通过降低过氧化 (H2O2) 水平来抑制大米种子的发芽,这与降低的超氧化物脱酶 (SOD) 活性有关. 这种效应进一步受到改变的吉伯酸/酸 (GA/ABA) 平衡和ABA信号通路的影响.

科学领域:

植物生理学植物生理学
分子生物学分子生物学
生物化学生物化学

背景情况:

在种子发芽研究中,乙醇被用作植物激素的溶剂,如吉伯酸 (GA) 和酸 (ABA).
乙醇对种子发芽的影响背后的精确分子机制尚未完全理解.

研究的目的:

调查乙醇在米种子发芽过程中对活性氧物种 (ROS) 和相关分子通路的影响.
阐明过氧化 (H2O2),抗氧化酶和植物激素平衡在乙醇诱导的发芽抑制中的作用.

主要方法:

米种子被用乙醇 (3.5%v/v) 和/或H2O2.2.处理.
测量结果包括种子发芽率,H2O2含量,超氧化解突变酶 (SOD) 活性和基因表达分析 (SOD,OsNCEDs,OsABA8ox3,ABA受体,ABRE结合因子).
在种子胚胎中量化了植物激素水平 (ABA,GA).

主要成果:

乙醇显著抑制了米种子的发芽,这种效应在很大程度上被H2O2.2.反转.
乙醇降低了H2O2水平,并抑制了胚胎中的SOD活性和基因表达.
乙醇治疗导致ABA增加和GA水平降低,破坏GA/ABA平衡.
乙醇调节了参与ABA生物合成和信号通路的关键基因.

结论:

由于抑制SOD活性而降低的H2O2水平是乙醇诱导的种子发芽抑制的主要原因.
破坏GA/ABA平衡和调节ABA信号通路显著导致乙醇对大米种子发芽的抑制作用.

关键词:

乙醇乙醇是一种在GA/ABA余额中.H ((2) O ((2)) 的情况.米树 (Oryza sativa) L. 米树 (Oryza sativa) L. 米树 (Oryza sativa) 是一个植物.这就是SOD的意义.种子的发芽种子的发芽

更多相关视频

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Published on: May 21, 2019

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

相关概念视频

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

Responses to Drought and Flooding

Responses to Drought and Flooding

Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...