在生物床中以微空气甲驱动的脱化 - 通过使用RNA稳定同位素探测来研究活跃的微生物生物膜社区组成
Emmanuel O Egbadon1, Kathryn Wigley1, Sunday T Nwoba1
1Department of Chemical & Process Engineering, University of Canterbury, Christchurch, New Zealand.
Chemosphere
|October 31, 2023
概括
这项研究使用RNA稳定同位素探测在甲驱动的酸盐去除系统中识别了活跃的微生物. 由甲氧化微生物为燃料的脱细菌主导了活跃的微生物群体.
科学领域:
- 环境微生物学环境微生物学
- 生物技术是生物技术.
- 生物地质化学生物地质化学
背景情况:
- 酸盐污染是一个重要的环境问题.
- 生物酸盐去除通常依赖于有机碳来源.
- 甲为脱提供了一个可持续的替代碳来源.
研究的目的:
- 为了识别微空气甲驱动的酸盐去除 (MAME-D) 生物床反应堆中的活跃微生物群体.
- 了解微生物在甲氧化和酸盐减少中的功能作用.
- 在这种类型的系统中首次应用RNA-稳定同位素探测 (RNA-SIP).
主要方法:
- 使用甲和酸盐运行微空气生物三床反应堆.
- 利用16S rDNA和16S rRNA扩增序列测序来描述总和活跃的微生物群落.
- 使用13CH4的RNA稳定同位素探测 (RNA-SIP) 来追踪活跃的微生物代谢.
主要成果:
- 甲和酸盐的去除速度持续55天.
- 16S rDNA分析显示,梅西洛辛斯,努贝塞拉,伪桑托蒙纳斯和奥斯库里细菌群是主导的种类.
- RNA-SIP揭示了Methylocystis,Methylosinus,Arenimonas,Pseudoxanthomonas和Obscuribacterales作为最活跃的属,表明甲氧化与脱结合.
结论:
- 在MAME-D反应堆中,活跃的微生物群落与整个群落不同.
- 甲型细菌为脱细菌提供了必不可少的中间体.
- 这项研究开创了RNA-SIP在微空气甲驱动的脱系统中的应用,揭示了关键的微生物参与者及其相互作用.
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