相关实验视频

Updated: Jul 12, 2025

Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues

Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues

Published on: May 8, 2016

外围辅助T细胞衍生CXCL13是异常多中心卡斯特曼病的关键病原性因素

Takuya Harada¹, Yoshikane Kikushige^1,2, Toshihiro Miyamoto³

¹Department of Medicine and Biosystemic Science, Kyushu University Graduate School of Medicine, Fukuoka, Japan.

Nature communications

|November 1, 2023

概括

异常多中心卡斯特曼病 (iMCD-NOS) 涉及产生CXCL13的Tph细胞,导致致命的炎症. 准CXCL13为这种罕见疾病提供了潜在的治疗策略.

科学领域:

免疫学免疫学免疫学
血液学血液学血液学
病变发生学研究病变发生学研究

背景情况:

卡斯特曼病 (CD) 是一种罕见的淋巴增殖性疾病.
异形多中心性CD-not otherwise specified (iMCD-NOS) 呈现出一个不良的预后与未知的病原体.
现有的模型不能完全捕捉iMCD-NOS病理.

研究的目的:

建立一个异种移植模型来研究iMCD-NOS的病原性.
为了确定iMCD-NOS炎症的关键细胞和分子驱动因素.
探索iMCD-NOS的潜在治疗点.

主要方法:

人类淋巴结细胞外移植到免疫缺陷小鼠体内.
来自人体移植的CD3+T细胞的耗尽.
在小鼠血清中量化人类CXCL13水平.
对人类CXCL13.13进行中和抗体的使用.

主要成果:

移植iMCD-NOS患者的淋巴结细胞在小鼠中诱导致命的iMCD类炎症.
炎症依赖于人类的CD3+ T细胞.
观察到外围辅助T (Tph) 细胞的扩张和血清人类CXCL13的升高.
反人类CXCL13抗体治疗改善了炎症和改善了生存率.

结论:

产生CXCL13的外围辅助T (Tph) 细胞在iMCD-NOS病变发生过程中至关重要.
这项研究确立了iMCD-NOS作为一种免疫调节障碍.
这种异种移植模型为进一步的iMCD-NOS研究和治疗开发提供了一个平台.

更多相关视频

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Published on: January 7, 2019

相关概念视频

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

Differentiation of Common Myeloid Progenitor Cells

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...