Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

23
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
23
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

24
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
24

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Spatial Transcriptomic Profiling Reveals Microenvironment-Dependent Immune Signatures in a Lyme Arthritis model.

bioRxiv : the preprint server for biology·2026
Same author

Effects of a Plant-Based Multi-Strain <i>Limosilactobacillus fermentum</i> Probiotic on Weight Loss Outcomes in Overweight and Obese Adults: A Preliminary Study.

Nutrients·2026
Same author

Efficacy of Human Milk Oligosaccharide 6'-Sialyllactose Supplementation on Exercise Performance and Training Adaptations.

Nutrients·2026
Same author

Outcomes of Nephrolithiasis Care by Patients' Race: From a Statewide Quality Improvement Collaborative.

Cureus·2026
Same author

Impact of Electron Beam (eBeam) Treatment on Meat Quality and Sensory Attributes of Ground Chicken and Turkey.

Poultry science·2026
Same author

Bacterial-viral conflicts shape cholera evolution.

Nature·2026

相关实验视频

Updated: Jul 12, 2025

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
08:03

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

2.2K

使用深度测序基因组监测数据推断细菌传播动态.

Madikay Senghore1, Hannah Read2, Priyali Oza2

  • 1Center for Communicable Disease Dynamics, Department of Epidemiology, Harvard TH Chan School of Public Health, Boston, MA, USA. msenghore@hsph.harvard.edu.

Nature communications
|November 1, 2023
PubMed
概括
此摘要是机器生成的。

使用深度测序追踪病原体多样性有助于识别传染病传播对. 主体内部单核酸变体 (iSNVs) 的等位基频率准确地推断出传播链接,揭示了瓶效应.

更多相关视频

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings
10:26

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

5.3K
Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
07:21

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing

Published on: August 25, 2018

12.9K

相关实验视频

Last Updated: Jul 12, 2025

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
08:03

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

2.2K
Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings
10:26

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

5.3K
Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
07:21

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing

Published on: August 25, 2018

12.9K

科学领域:

  • 微生物学 微生物学
  • 基因组学就是基因组学.
  • 流行病学 流行病学

背景情况:

  • 识别传染病传播链对于控制至关重要.
  • 主体内病原体变异是推断传播事件的未充分利用的标记.
  • 存在有限的数据集,捕捉了捐赠者和接受者宿主中的病原体多样性.

研究的目的:

  • 评估深度测序基因组监测对识别传播对的有用性.
  • 开发一种模型,根据病原体遗传变异推断传播可能性.
  • 调查宿主内部变异和传输瓶的作用.

主要方法:

  • 利用小鼠模型对Citrobacter rodentium的受控传播进行了研究.
  • 采用深度测序基因组监测来分析病原体多样性.
  • 检查了宿主内部的单核酸变异 (iSNVs) 和它们的等位素频率.

主要成果:

  • 宿主内部单核酸变体 (iSNVs) 在多个传播步骤中保持.
  • 开发了一个模型,使用iSNV数据推断传输对的概率.
  • 对iSNVs的等位基频率差异提供了比单独的存在/缺席更精确的传输对推断.
  • 传输瓶被证明在保持宿主内部多样性方面发挥着关键作用.

结论:

  • 深度测序基因组监测对于识别病原体传播对是有效的.
  • 主体内部变异的等位频率提供了一个精确的方法来推断传输链接.
  • 了解传播期间的病原体多样性动态对于疾病控制策略至关重要.