细胞外酶的生产由Lentinula edodes菌株在固态发酵中的Lignocellulosic生物质上通过物理和化学方法灭菌
Ezequiel Marcelino Silva1, Adriane Maria Ferreira Milagres2
1Department of Biotechnology, Fundação Universidade Federal do Tocantins, Gurupi, TO, CEP 77 402 970, Brazil. ezequielsilva@uft.edu.br.
Current microbiology
|November 1, 2023
概括
使用过氧化 (H2O2) 来化学杀菌细胞生物质,增强了Lentinula edodes的细胞外酶生产. 这种方法更快,需要更少的能量,和避免有毒的副产品相比自动.
科学领域:
- 生物技术是生物技术.
- 酶技术 酶技术是一种
- 生物质杀菌生物质杀菌
背景情况:
- 细胞生物质是微生物酶生产的关键基质.
- 绝育方法会影响酶产量和微生物生长.
- 传统的物理灭菌 (自闭式灭菌) 可能耗费大量的能量和时间.
研究的目的:
- 为了比较化学 (H2O2) 与物理 (自闭式) 灭菌的效果,用于酶生产的林氏纤维素生物质.
- 评估绝育方法对Lentinula edodes产生的细胞外酶概况的影响.
- 优化生物质灭菌以提高酶产量.
主要方法:
- 树废弃物 (EW) 和大米 (RB) 混合物 (80:20和90:10) 通过自封和过氧化 (H2O2) 进行了灭菌.
- 在大米中,用250毫米的H2O2.2.实现了catalase无活化.
- 通过Lentinula edodes菌株量化细胞外酶的产生,并比较了杀菌方法.
主要成果:
- 使用H2O2进行化学杀菌显著有利于更高的水解酶的产生,包括内葡萄糖酶,β-葡萄糖酶和西兰酶.
- 氧化酶生产 (MnP,laccase) 在菌株和灭菌方法之间有所不同,使用H2O2的laccase和MnP的生产时间较短.
- 通过H2O2灭菌,可以获得更高的关键酶产量,如西兰酶 (55,000 IU/kg) 和β-葡萄糖酶 (1,000 IU/kg).
结论:
- 使用过氧化进行化学灭菌是一种优越的方法,可以增强从细胞生物质中提升细胞外酶的产生.
- 与自动封装相比,H2O2方法具有降低能源消耗和缩短处理时间的优势.
- 过氧化灭菌可以避免形成抑制性化合物,如furfural和hydroxy-methyl-furfural,促进最佳的微生物和酶活性.
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