流马尔迪显微镜:矩阵联合结晶同时增强光和马尔迪成像
Ethan Yang1,2, Xinyi Elaine Shen1,2, Hoku West-Foyle3,4
1Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|November 1, 2023
概括
与矩阵辅助激光脱/电离 (MALDI) 成像矩阵共同结晶的光体增加了亮度,使FluoMALDI. 这种技术结合了光显微镜和MALDI成像,用于增强生物样本分析.
科学领域:
- 分析化学 分析化学
- 生物医学成像技术 生物医学成像技术
- 显微镜的使用方法
背景情况:
- 矩阵辅助激光脱吸/电离 (MALDI) 成像提供了多功能,多重复合的能力.
- 光显微镜提供高空间分辨率和特定的标签.
- 由于样本操纵和注册问题,整合这些模式具有挑战性.
研究的目的:
- 开发一种新的成像技术,FluoMALDI,通过与MALDI矩阵共同结晶光体.
- 为了增强光体的亮度和放大组织自光,以改善成像.
- 通过光显微镜和MALDI成像,使完全相同的生物样本能够无成像.
主要方法:
- 用MALDI成像矩阵进行光体的联合结晶.
- 用FluoMALDI应用于内源性和外源性光体.
- 在大脑和脏组织部位上展示基于自光的FluoMALDI.
主要成果:
- 通过联合结晶,光体的亮度提高了79倍.
- 在相同的组织部位上,FluoMALDI成功地将光显微镜和MALDI成像技术结合起来.
- 该方法允许在没有物理样本变化的情况下对两种模式中的相同细胞进行成像.
- 对内源性和外源性光体以及自光体的可行性得到证明.
结论:
- FluoMALDI代表了相关成像技术的重大进步,融合光和MALDI技术.
- 增强的亮度和简化的注册可以促进结构功能显微镜成像.
- 这种技术对细胞生物学,生物医学和病理学中的应用具有前景,特别是对于具有低光度的样本.
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