八胺素A的生物合成:不常见的启动/扩展单元和产品通过分子间化释放
Yanghui Liao1, Xue-Jiao Wang1, Guang-Lei Ma2
1School of Biological Sciences, Nanyang Technological University, Singapore, 67551, Singapore.
Chembiochem : a European journal of chemical biology
|November 1, 2023
概括
链杆菌素触发了Amycolatopsis azurea中抗真菌性octacosamicin A的产生. 在这种情况下,链杆菌素会引发抗真菌性octacosamicin A的产生. 研究人员确定了对其独特的多基酸合成酶组合和非传统的甘氨酸附着负责的基因群.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 自然产品化学 自然产品化学
背景情况:
- 八胺素A是一种强大的抗真菌代谢物,具有线性聚烯-聚醇链的特点.
- 它的结构包括N-hydroxyguanidine和glycine部分,表明一个复杂的生物合成途径.
- 了解这些天然产品的生物合成对于发现新的抗菌剂至关重要.
研究的目的:
- 为了研究通过低抑制度的 estreptomycin.elicitation产生的octacosamicin A 的产生.
- 为了识别和表征生物合成基因集群 (oca BGC) 负责octacosamicin A合成.
- 阐明聚-聚醇链组装的基础机制和独特部分的结合.
主要方法:
- 艾米科拉托普西斯青菌的培养 DSM 43854T与亚抑制性链杆菌素.
- 鉴定和分析八胺素A生物合成基因集群 (oca BGC).
- 模块化聚基合成酶 (PKS) 系统和相关酶的生物信息分析.
主要成果:
- 发现亚抑制性链杆菌素度诱导了青性 Amycolatopsis 中的 octacosamicin A 生产.
- 确定了oca BGC,它编码了一个负责聚-聚醇链组件的模块化PKS系统.
- N-基瓜尼丁部分很可能来自于4-基基甲基启动剂,并且通过 (2R) - 基基甲基扩展剂将α-基基纳入.
- 一个非正规的终端模块缺少铁酶和乙烯载体蛋白域,表明一种非常规的甘氨酸附着机制.
结论:
- 斯特雷普托米辛作为一个催化剂,用于Amycolatopsis azurea中的octacosamicin A生产.
- 生物合成涉及一个独特的PKS组装途径和一个神秘的分子间化,用于糖氨酸的整合.
- 这项研究为复杂的多聚多聚天然产品的生物合成提供了重要的见解.
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