伪虫光体 MFE01使用1-undecene作为航空通信分子
Charly A Dupont1,2, Yvann Bourigault1,2, Théo Osmond1,2
1Laboratoire de Communication Bactérienne et Stratégies Anti-infectieuses (CBSA UR), Univ Rouen Normandie, Université Caen Normandie, Normandie Univ, Rouen, France.
Frontiers in microbiology
|November 1, 2023
概括
形光菌MFE01使用1-undecene,一种挥发性有机化合物,用于空中通信. 这种细菌信号对于生物膜成熟和协调集体行为至关重要.
科学领域:
- 微生物学 微生物学
- 细菌的传播 细菌的传播
- 化学生态化学生态学
背景情况:
- 细菌的交流同步了基因表达和集体行为.
- 多数感应,通常由N-acyl homoserine乳介导,在Pseudomonas中得到了很好的研究.
- 挥发性有机化合物 (VOCs) 作为通信信号,特别是在根球中,还没有得到充分的研究.
研究的目的:
- 为了研究1-undecene的作用,一种由Pseudomonas fluorescens MFE01发出的VOC,在细菌通信中.
- 了解1-undecene对MFE01协调表型的影响,包括六型分泌系统活动和生物膜形成.
- 为了确定P. fluorescens MFE01是否使用VOC进行种内交流.
主要方法:
- 在1-undecene合成中缺乏突变菌株 (ΔundA) 的构建.
- 使用头部空间固态微提取和气体染色体质谱法分析挥发性有机物排放.
- 评估空中抑制莱吉欧内拉肺炎菌生长和对MFE01生物膜成熟的影响.
主要成果:
- 突变的DundA表现出受损的生物膜成熟.
- 对DundA突变体暴露于MFE01发射的VOC恢复了生物膜成熟.
- P. fluorescens MFE01缺乏N-类荷马酸乳合成基因,并且不受N-类荷马酸乳的抑制.
结论:
- 在P. fluorescens MFE01.01中,1-undecene作为一个空中通信信号.
- 这项研究确定了1-undecene作为一种新型细菌物种内特异性通信信号.
- 在P. fluorescens MFE01的集体行为中,VOC介导的通信起着重要作用.
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