在宫动脉剖析患者中,循环RNA的表达变化
Yifan Wang1, Zhaofei Dong2, Jie Li1
1Department of Neurology, Shenzhen Hospital, Southern Medical University, Shenzhen, Guangdong, China.
Frontiers in neurology
|November 1, 2023
概括
异常的循环RNA (circRNA) 表达与宫动脉剖析 (CeAD) 有关,这是年轻人中风的主要原因. 这些circRNAs可以作为CeAD的诊断生物标志物和治疗点.
科学领域:
- 神经科学是一个神经科学.
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
背景情况:
- 宫动脉解剖 (CeAD) 是一种脑血管疾病,也是年轻人中风的主要原因,经常导致误诊.
- 循环RNAs (circRNAs) 是参与基因调节的非编码RNA,已知与神经系统疾病有关,但它们在CeAD中的作用尚不清楚.
研究的目的:
- 研究circRNAs在CeAD病理生理学中的作用.
- 为了确定CeAD诊断的潜在circRNA生物标志物.
主要方法:
- 来自CeAD患者和健康对照的外周血液单核细胞的高通量测序.
- 生物信息学分析以确定差异表达的circRNA和相关途径.
- 定量实时PCR (qRT-PCR) 用于验证关键的circRNAs.
主要成果:
- 与对照组相比,CeAD患者中发现了460个差异表达的circRNAs (p <0.05,折叠变化≥2).
- 有240个circRNA被上调,220个circRNA被下调.
- 三种circRNA在qRT-PCR验证时显示出一致的差异表达,并与蛋白质代谢,突触功能和CeAD相关的炎症途径有关.
结论:
- 异常表达的circRNA与CeAD有关.
- 这些circRNA可以作为CeAD的有价值的诊断生物标志物.
- 循环RNAs代表了管理CeAD及其相关中风并发症的潜在治疗点.
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