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异质的小鼠周腺组织分隔的上皮细胞更新

Serrena Singh¹, Qiuyu Lian², Tifanny Budiman¹

¹Section of Digestive Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA.

Developmental cell

|November 1, 2023

概括

肝外胆道具有独特的细胞群,可以更新组织. 坑细胞更新表面,而基底细胞维持腺体或表面,揭示了细胞层次结构.

科学领域:

肝胆系统研究研究肝胆系统研究
细胞生物学和组织稳态.
胃肠道上皮的更新

背景情况:

肝外胆道树的表面上皮质更新机制尚未完全理解.
负责维持胆道表皮的特定细胞类型仍然未确定.

研究的目的:

识别和描述负责恒常更新肝外胆道表皮的细胞.
阐明细胞层次和分化途径涉及胆道上皮质维护.

主要方法:

在小鼠中无标记物和有针对性的克隆命运映射.
单细胞RNA测序 (scRNA-seq) 用于识别细胞特异性基因表达.
在生理变化 (禁食/再养) 和基因操纵 (Wnt信号) 下对上皮质可塑性的分析.

主要成果:

肝外胆道表皮是分隔的,具有不同的细胞群.
来自外壁腺体的皮坑胆细胞更新了表面上皮质.
基底导向的胆血管细胞维持腺体或更新壁状腺体的表面上皮质.
单细胞测序揭示了基底和表面区间之间的分级基因表达.
表皮细胞表现出可塑性,在禁食和再养时改变身份.
激活Wnt信号导致基底细胞扩张和改变标记物表达.

结论:

一个细胞层次统治肝外胆膜上皮的更新.
特定的胆管细胞群 (和基底) 负责保持胆管完整性.
胆道上皮表现出可塑性,适应生理条件和信号通路.

关键词:

一个Lgr55,一个Lgr5 这就是 Muc6 的原因.他们不想做.胆汁腺是什么意思胆汁腺是什么意思胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃肠胃恒常状态 (homeostasis) 是一种平衡状态.肝脏肝脏肝脏肝脏肝脏肝脏干细胞是干细胞的组成部分.

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